Phenotyping Tumor-Associated Macrophages.

Multi-parametric flow cytometry of tumor-bearing murine nonlymphoid tissue allows for characterization, isolation, and examination of immune cell composition, phenotype, and function. Here we describe an approach to process nonlymphoid tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This panel can be used to phenotype tumor-associated macrophages.