Bénard Camille, Acket Sébastien, Rossez Yannick, Fernandez Olivier, Berton Thierry, Gibon Yves, Cabasson Cécile
Service de Pharmacologie et Immunoanalyse (SPI), MetaboHUB-Paris, CEA, INRA, Université Paris Saclay, Gif-sur-Yvette Cedex, France.
UMR 1332 Biologie du Fruit et Pathologie, Centre INRA de Nouvelle Aquitaine-Bordeaux, INRA, Univ. Bordeaux, Villenave d'Ornon, France.
Methods Mol Biol. 2018;1778:101-124. doi: 10.1007/978-1-4939-7819-9_8.
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described.
植物的种植方式以及样本的收获、制备和提取方法对代谢组学实验的结果有着深远影响。在本章中，我们详细介绍了从植物栽培到提取物制备的实验步骤，以避免可能导致分析物污染或意外变化的问题。文中以两种植物器官为例：番茄果实（番茄）和亚麻籽（亚麻）。还描述了用于通过液相色谱 - 质谱联用（LC-MS）对半极性化合物进行非靶向分析以及通过气相色谱 - 质谱联用（GC-MS）或带有火焰离子化检测器的气相色谱（GC-FID）对脂肪酸进行靶向分析的提取方法。
