Boughton Berin A, Thinagaran Dinaiz
Metabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2018;1778:241-252. doi: 10.1007/978-1-4939-7819-9_17.
Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.
质谱成像（MSI）是一种用于测量组织中多种生物分子时空分布的新兴技术。在过去十年中，MSI已被植物生物学家采用，并应用于广泛的领域，包括：初级代谢、天然产物、植物防御、植物对非生物和生物胁迫的反应、植物脂质以及空间代谢组学这一新兴领域。本章方法涵盖了用于基质辅助激光解吸电离（MALDI）-MSI的植物组织制备，包括样品包埋和冷冻、切片、固定以及在MSI分析之前使用升华和喷雾沉积进行基质沉积。
