Wataya Y, Hazawa T, Watanabe K, Hirota Y, Yoshioka-Hiramoto A
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Nucleic Acids Symp Ser. 1988(19):53-5.
The mechanism of intracellular deoxyribonucleoside-triphosphate (dNTP) imbalance death of mouse mammary tumor FM3A cells was studied. When the cells were exposed to 5-fluorodeoxyuridine, deoxyadenosine, or 2-chlorodeoxyadenosine, dNTP pool imbalance resulted. The imbalance was followed by DNA double strand breaks and subsequent cell death. The DNA double strand breaks have been directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). Fragmented DNA band appeared to be approximately 100-200 kb in size.
研究了小鼠乳腺肿瘤FM3A细胞的细胞内脱氧核糖核苷三磷酸（dNTP）失衡死亡机制。当细胞暴露于5-氟脱氧尿苷、脱氧腺苷或2-氯脱氧腺苷时，会导致dNTP库失衡。失衡之后是DNA双链断裂及随后的细胞死亡。已通过正交交变电场凝胶电泳（OFAGE）直接检测了DNA双链断裂。断裂的DNA条带大小似乎约为100-200 kb。
