Liu Xiao, Luo Yufu, Zhang Yanjun
China National Academy of Nanotechnology & Engineering, Tianjin 300457, P.R.China.
Tianjin Yongjiu Hospital, Tianjin 300450, P.R.China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2017 Aug 1;34(5):767-771. doi: 10.7507/1001-5515.201611004.
Platelets are rapidly activated by activators and produce a large number of platelet microparticles (PMPs) with high coagulation activity, resulting in coagulation dysfunction. However, the generation mechanism of PMPs is still not clear. Hopping probe ion conductance microscopy (HPICM) has special technical advantages in non-contact, real-time, high-resolution imaging of living cells under physiological conditions. Using HPICM, this study monitored the processes of platelet activation and generation of PMPs in real time in the presence of calcium ionophore A23187 and cytochalasin D (CD), respectively. The results proved that the intracellular calcium concentration and the cytoskeletal proteins played important roles in the platelet activation and the generation of PMPs. Compared with the low density spread shape platelets (LDSS), the high density bubble shape platelets (HDBS) were more sensitive to the calcium ionophore A23187 and cytochalasin D. This research has a guiding significance for the further study on the relationship between platelet activation and coagulation function using HPICM.
血小板被激活剂迅速激活，并产生大量具有高凝血活性的血小板微粒（PMPs），导致凝血功能障碍。然而，PMPs的产生机制仍不清楚。跳跃探针离子电导显微镜（HPICM）在生理条件下对活细胞进行非接触、实时、高分辨率成像方面具有特殊的技术优势。本研究利用HPICM分别在钙离子载体A23187和细胞松弛素D（CD）存在的情况下实时监测血小板激活和PMPs产生的过程。结果证明，细胞内钙浓度和细胞骨架蛋白在血小板激活和PMPs产生中起重要作用。与低密度铺展形血小板（LDSS）相比，高密度泡状血小板（HDBS）对钙离子载体A23187和细胞松弛素D更敏感。本研究对利用HPICM进一步研究血小板激活与凝血功能之间的关系具有指导意义。
