环状 RNA_0023642 通过调控 EMT 促进胃癌细胞的迁移和侵袭。

CircRNA_0023642 promotes migration and invasion of gastric cancer cells by regulating EMT.

机构信息

Digestive Endoscopy Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Apr;22(8):2297-2303. doi: 10.26355/eurrev_201804_14818.

DOI:10.26355/eurrev_201804_14818

PMID:29762831

Abstract

OBJECTIVE

Non-coding circular RNAs (circRNAs) have displayed dysregulated expression in various tumor tissues. However, their role in the progression of cancers remains largely unknown. We aimed at examining the expression, functions, and molecular mechanisms of a new circRNA (circRNA_0023642) in gastric cancer (GC).

PATIENTS AND METHODS

We evaluated the expression levels of circRNA_0023642 in GC tissues, adjacent normal tissues and cells lines using qRT-PCR. The functional roles of circRNA_0023642 in GC were determined by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, transwell assay, and flow cytometric analysis. Western blot was used to analyze the effect of circRNA_0023642 on the expression of EMT-related proteins.

RESULTS

We found that circRNA_0023642 was upregulated in GC tissues and cell lines. Functionally, down-regulation of circRNA_0023642 displayed the tumor-inhibitory effects by suppressing cell proliferation, migration, and invasion as well as inducing apoptosis. Mechanically, our results revealed that the abnormal expression of circRNA_0023642 could influence the EMT signaling pathway, which was demonstrated by measuring the expression levels of N-cadherin, vimentin snail, and E-cadherin.

CONCLUSIONS

Our findings suggest that circRNA_0023642 serves as a metastasis activator by promoting EMT and may represent a novel molecular therapeutic target for GC.

摘要

目的

非编码环状 RNA（circRNAs）在各种肿瘤组织中表现出失调表达。然而，它们在癌症进展中的作用在很大程度上仍然未知。我们旨在研究一种新的环状 RNA（circRNA_0023642）在胃癌（GC）中的表达、功能和分子机制。

患者和方法

我们使用 qRT-PCR 评估了 GC 组织、相邻正常组织和细胞系中 circRNA_0023642 的表达水平。通过细胞计数试剂盒-8（CCK-8）测定、集落形成测定、transwell 测定和流式细胞术分析确定 circRNA_0023642 在 GC 中的功能作用。Western blot 用于分析 circRNA_0023642 对 EMT 相关蛋白表达的影响。

结果

我们发现 circRNA_0023642 在 GC 组织和细胞系中上调。功能上，circRNA_0023642 的下调通过抑制细胞增殖、迁移和侵袭以及诱导细胞凋亡表现出肿瘤抑制作用。机制上，我们的结果表明，circRNA_0023642 的异常表达可以影响 EMT 信号通路，这可以通过测量 N-钙粘蛋白、波形蛋白、snail 和 E-钙粘蛋白的表达水平来证明。