[使用胎盘细胞核进行体外转录技术的实验条件]
[Experimental conditions of a technic of in vitro transcription using placental nuclei].
作者信息
Hess K, el Ouardirhi H, Belleville F, Paysant P, Nabet P
机构信息
Laboratoire de Biochimie Médicale I, Faculté de Médecine, Vandaeuvre-les-Nancy.
出版信息
C R Seances Soc Biol Fil. 1988;182(3):286-95.
PMID:2976296
Abstract
To examine the expression of human placental lactogen, we investigated transcription in isolated placental nuclei. Using SH-agarose affinity chromatography, we have isolated Hg-RNA synthetized in vitro. These RNA are translated in a wheat germ cell-free system. hPL synthetized in vitro, accounting for 20% of total protein. This system should prove useful for the study of gene expression of hPL.
摘要
为检测人胎盘催乳素的表达，我们研究了分离的胎盘细胞核中的转录情况。利用SH-琼脂糖亲和层析法，我们分离出了体外合成的汞RNA。这些RNA在无细胞小麦胚芽体系中进行翻译。体外合成的人胎盘催乳素占总蛋白的20%。该体系对研究人胎盘催乳素的基因表达应会很有用。
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