大鼠心肌细胞核膜中的单通道离子电流

SINGLE-CHANNEL ION CURRENTS IN THE NUCLEAR ENVELOPE OF RAT CARDIOMYOCYTES.

作者信息

Kotyk O A, Kotliarova A B, Polishchuk A O, Marchenko S M

出版信息

Fiziol Zh (1994). 2016;62(6):3-8. doi: 10.15407/fz62.06.003.

DOI:10.15407/fz62.06.003

Abstract

Using the patch clamp technique in nucleus attached configuration we have found that the nuclear membrane of rat cardiomyocytes contains different types of ion channels with conductances in the range from 10 to 400 pS. In particular, we recorded inositol 1,4,5-trisphosphate receptors with conductance of 384 ± 5 pS and 209 ± 13 pS cation channels similar to LCC-channels, previously reported in neurons. In addition, we found at least two types of ion channels with significantly higher conductance than that of LCC-channels and several types of ion channels with lower conductance (10-90 pS).

摘要

采用细胞核贴附式膜片钳技术，我们发现大鼠心肌细胞的核膜含有不同类型的离子通道，其电导范围为10至400 pS。特别地，我们记录到了电导为384±5 pS的肌醇1,4,5 - 三磷酸受体以及电导为209±13 pS的阳离子通道，这些阳离子通道类似于先前在神经元中报道的LCC通道。此外，我们发现至少有两种类型的离子通道，其电导显著高于LCC通道，还有几种类型的离子通道电导较低（10 - 90 pS）。