Choi B H
Division of Neuropathology, University of California, Irvine 92717.
Glia. 1988;1(5):308-16. doi: 10.1002/glia.440010503.
A correlative light microscopic, ultrastructural, and immunocytochemical study was made of the brains of C57BL/6J mice obtained between embryonic day (E-) 11 to postnatal day (P-) 3. The deployment of radially oriented glial cells within the neopallium was already apparent by E-12, at which time the expanded endfeet of processes abutting the basement membrane at the pial surface showed ultrastructural evidence of glial differentiation. Scattered, horizontally arranged glial cells were also observed within the marginal zone prior to the arrival of the cortical plate neurons. Indirect immunofluorescence for glial fibrillary acidic protein (GFAP) was detected at the outer margins of the neopallium at E-12, antedating the expression of GFAP in radially organized fibers. Radial fibers traversing the full thickness of the neopallium became strongly immunoreactive for GFAP only at and after day E-16. These findings provide evidence of early gliogenesis in the developing mouse forebrain and indicate that GFAP is as readily demonstrable in mouse astrocytes and radial glial fibers as it is in those of man and subhuman primates.
对胚胎期第11天（E-11）至出生后第3天（P-3）的C57BL/6J小鼠大脑进行了相关的光学显微镜、超微结构和免疫细胞化学研究。在E-12时，新皮层内呈放射状排列的神经胶质细胞的分布已很明显，此时，在软膜表面与基底膜相邻的突起的扩张终足显示出神经胶质细胞分化的超微结构证据。在皮质板神经元到达之前，在边缘区内也观察到散在的、水平排列的神经胶质细胞。在E-12时，在新皮层的外缘检测到胶质纤维酸性蛋白（GFAP）的间接免疫荧光，早于GFAP在放射状组织纤维中的表达。仅在E-16天及之后，穿过新皮层全层的放射状纤维对GFAP才具有强免疫反应性。这些发现为发育中小鼠前脑早期神经胶质细胞生成提供了证据，并表明GFAP在小鼠星形胶质细胞和放射状神经胶质纤维中与在人类和亚人类灵长类动物中一样易于显示。
