Interfaculty Institute of Biochemistry (IFIB), Eberhard Karls Universität Tübingen, Germany.
FEBS Lett. 2018 Jun;592(11):1870-1881. doi: 10.1002/1873-3468.13098. Epub 2018 May 28.
Localization of mRNAs depends on specific RNA-binding proteins (RBPs) and critically contributes not only to cell polarization but also to basal cell function. The yeast RBP Khd1p binds to several hundred mRNAs, the majority of which encodes secreted or membrane proteins. We demonstrate that a subfraction of Khd1p associates with artificial liposomes and endoplasmic reticulum (ER), and that Khd1p endomembrane association is partially dependent on its binding to RNA. ER targeting of at least two mRNAs, MID2 and SLG1/WSC1, requires KHD1 but is independent of their translation. Together, our results suggest interdependence of Khd1p and mRNA for their targeting to the ER and presents additional evidence for signal sequence-independent, RBP-mediated mRNA targeting.
mRNA 的定位取决于特定的 RNA 结合蛋白（RBPs），不仅对细胞极化至关重要，而且对基础细胞功能也至关重要。酵母 RBP Khd1p 与几百个 mRNA 结合，其中大多数编码分泌蛋白或膜蛋白。我们证明，Khd1p 的亚部分与人工脂质体和内质网（ER）结合，并且 Khd1p 内质网的结合部分依赖于其与 RNA 的结合。至少两种 mRNA（MID2 和 SLG1/WSC1）的 ER 靶向需要 KHD1，但不依赖于它们的翻译。总之，我们的结果表明 Khd1p 和 mRNA 之间存在相互依赖关系，以便将它们靶向到 ER，并为信号序列非依赖性、RBP 介导的 mRNA 靶向提供了额外的证据。
