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Coliphage lambda to terminator lowers the stability of messenger RNA in Escherichia coli hosts.

作者信息

Gross G, Hollatz I

机构信息

Gesellschaft für Biotechnologische Forschung, Abteilung Genetik, Braunschweig, F.R.G.

出版信息

Gene. 1988 Dec 10;72(1-2):119-28. doi: 10.1016/0378-1119(88)90133-3.

DOI:10.1016/0378-1119(88)90133-3
PMID:2977353
Abstract

The effects of the transcription terminators to and tfd on the overall high-level expression of a human interferon-beta gene (IFN-beta) in Escherichia coli hosts were compared. Deletion mapping shows that mRNA lability is caused by sequences at or near the lambda terminator to stem-loop structure. Extensive RNA secondary structure in this region indicates a potential RNase III cleavage/binding site. In RNase III- E. coli hosts, IFN-beta synthesis is indeed considerably enhanced. The bacteriophage tfd terminator does not confer this mRNA labilization phenomenon. In all cases, RNA level and stability correlate with the level of IFN-beta synthesized in the cell. In the system described, ongoing translation stabilizes mRNA only moderately.

摘要

相似文献

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