Rogers Matthew, Sobolik Tammy, Schaffer David K, Samson Philip C, Johnson Andrew C, Owens Philip, Codreanu Simona G, Sherrod Stacy D, McLean John A, Wikswo John P, Richmond Ann
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Biomicrofluidics. 2018 May 7;12(3):034102. doi: 10.1063/1.5016433. eCollection 2018 May.
The interaction of cancer cells with the stromal cells and matrix in the tumor microenvironment plays a key role in progression to metastasis. A better understanding of the mechanisms underlying these interactions would aid in developing new therapeutic approaches to inhibit this progression. Here, we describe the fabrication of a simple microfluidic bioreactor capable of recapitulating the three-dimensional breast tumor microenvironment. Cancer cell spheroids, fibroblasts, and endothelial cells co-cultured in this device create a robust microenvironment suitable for studying in real time the migration of cancer cells along matrix structures laid down by fibroblasts within the 3D tumor microenvironment. This system allows for ready evaluation of response to targeted therapy.
癌细胞与肿瘤微环境中的基质细胞和基质之间的相互作用在转移进展中起着关键作用。更好地理解这些相互作用背后的机制将有助于开发新的治疗方法来抑制这种进展。在此，我们描述了一种能够重现三维乳腺肿瘤微环境的简单微流控生物反应器的制造方法。在该装置中共培养的癌细胞球、成纤维细胞和内皮细胞创造了一个强大的微环境，适合实时研究癌细胞沿着三维肿瘤微环境中成纤维细胞铺设的基质结构的迁移。该系统便于评估对靶向治疗的反应。
