Kemah Bahattin, Uzer Gökçer, Turhan Yalçın, Özturan Burak, Kılıç Bülent, Gültepe Bilge Sümbül, Ceyran Ayşe Bahar, Ertürk Selim, Aksoylu Burak, Şenaydın Özlem, Özkan Korhan
Orthopedics and Traumatology Department, Ağrı State Hospital, Ağrı, Turkey.
Orthopedics and Traumatology Department, Bezmialem University Medical Faculty, Istanbul, Turkey.
J Bone Jt Infect. 2018 Apr 12;3(1):43-49. doi: 10.7150/jbji.22121. eCollection 2018.
: Bone and soft tissue infections are among the least desired complications after orthopaedic surgery. This study analysed the effects of the local application of nano-silver particles (AgNPs) [1nm = 1 billionth of a meter] in soft tissue infections. : An experimental osteomyelitis model was formed by inoculating both tibias of 24 rats with methicillin-resistant . The rats were followed without treatment for 21 days. Blood samples and tibial x-rays at day 21 confirmed the development of infection. Then, the rats were divided randomly into two groups. One group (12 rats) underwent surgical debridement and received 21 days of teicoplanin therapy. The second group had the same treatment, with the addition of local nano-silver. All of the rats were sacrificed at day 42. Blood and wound swab samples were taken and the culture results were analysed. : No differences were observed between the groups in healing values at pathological examination, or in changes in the number of colonies at days 21 and 42. No differences in white blood cell count (WBC) were observed between the groups before and after the treatment. : Although studies suggest the effectiveness of AgNPs on pathogens, we found that the application of nano-silver did not make any difference when used in addition to the classical osteomyelitis treatment with antibiotics and local surgical debridement. We believe that additional studies using repeated nano-silver application could be beneficial.
骨与软组织感染是骨科手术后最不愿出现的并发症之一。本研究分析了纳米银颗粒（AgNPs）[1纳米 = 一米的十亿分之一]局部应用于软组织感染的效果。：通过给24只大鼠的双侧胫骨接种耐甲氧西林菌建立实验性骨髓炎模型。大鼠未经治疗随访21天。第21天采集血样并进行胫骨X光检查，以确认感染的发生。然后，将大鼠随机分为两组。一组（12只大鼠）接受手术清创并接受21天的替考拉宁治疗。第二组接受相同治疗，并额外局部应用纳米银。所有大鼠在第42天处死。采集血样和伤口拭子样本并分析培养结果。：在病理检查的愈合值方面，以及在第21天和第42天菌落数量的变化方面，两组之间均未观察到差异。治疗前后两组的白细胞计数（WBC）也未观察到差异。：尽管有研究表明AgNPs对病原体有效，但我们发现，在使用抗生素和局部手术清创进行经典骨髓炎治疗的基础上额外应用纳米银并没有产生任何差异。我们认为，进一步开展重复应用纳米银的研究可能会有帮助。
