正常血压和高血压大鼠脊髓中的心房利钠因子

Atrial natriuretic factor in the spinal cord of normotensive and hypertensive rats.

作者信息

Debinski W, Kuchel O, Buu N T, Thibault G

机构信息

Clinical Research Institute of Montreal, Laboratory of the Autonomic Nervous System, Québec, Canada.

出版信息

Peptides. 1988 Sep-Oct;9(5):1101-5. doi: 10.1016/0196-9781(88)90096-4.

DOI:10.1016/0196-9781(88)90096-4

PMID:2977435

Abstract

Atrial natriuretic factor (ANF) was investigated in the rat spinal cord and hypothalamus using two radioimmunoassays. ANF was also quantified in both tissues of Spontaneously Hypertensive Rats and Dahl rats. Spinal cord and hypothalamus were found to be immunoreactive to proANF and its near-NH2- or near-COOH-terminal fragments. A major part of the extracted ANF was a COOH-terminal peptide smaller than or the same as ANF (Ser99-Tyr 126). SHR had higher hypothalamic and spinal cord ANF concentrations than Wistar Kyoto rats, while the Dahl salt-sensitive animals exhibited an increase in spinal cord ANF when compared with the Dahl salt-resistant group. The data suggest that spinal cord may produce ANF locally with processing similar to that in hypothalamus. Changes in ANF concentrations occurring during the course of hypertension remain to be further investigated.

摘要

使用两种放射免疫分析法对大鼠脊髓和下丘脑进行了心房利钠因子（ANF）的研究。还对自发性高血压大鼠和 Dahl 大鼠的这两种组织中的 ANF 进行了定量分析。发现脊髓和下丘脑对前 ANF 及其近氨基末端或近羧基末端片段具有免疫反应性。提取的 ANF 的主要部分是一种羧基末端肽，其大小小于或等于 ANF（Ser99-Tyr126）。与 Wistar Kyoto 大鼠相比，自发性高血压大鼠的下丘脑和脊髓 ANF 浓度更高，而与 Dahl 盐抵抗组相比，Dahl 盐敏感动物的脊髓 ANF 有所增加。数据表明脊髓可能在局部产生 ANF，其加工过程与下丘脑相似。高血压过程中 ANF 浓度的变化仍有待进一步研究。