全基因组关联研究揭示了绵羊（Ovis aries）耳朵大小的新基因。

Genome-wide association study reveals novel genes for the ear size in sheep (Ovis aries).

机构信息

Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, China.

State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, China.

出版信息

Anim Genet. 2018 Aug;49(4):345-348. doi: 10.1111/age.12670. Epub 2018 May 18.

DOI:10.1111/age.12670

PMID:29774586

Abstract

Variations in ear size can be observed in livestock such as sheep; however, the genetic basis of variable ear size in sheep is still poorly understood. To investigate causative genes associated with ear size in sheep, a genome-wide association study was performed in 115 adult Duolang sheep with different-sized floppy ears using the Ovine Infinium HD BeadChip. We found 38 significant SNPs at the genome-wide or chromosome-wise 5% significance level after Bonferroni correction. The most significant association (P = 1.61 × 10 ) was found at SNP rs402740419, located in the DCC gene, which plays a critical role in ear development. Also, we observed two additional significant SNPs, rs407891215 in PTPRD and rs407769095 in SOX5, both of which are functionally associated with ear developmental processes. Our results are useful for future sheep breeding and provide insights into the genetic basis of ear size development in sheep and other livestock.

摘要

耳朵大小的变化在绵羊等家畜中可以观察到；然而，绵羊耳朵大小变化的遗传基础仍知之甚少。为了研究与绵羊耳朵大小相关的致病基因，对 115 只具有不同大小的松软耳朵的多郎羊进行了全基因组关联研究，使用了绵羊 Infinium HD BeadChip。在经过 Bonferroni 校正后，我们在全基因组或染色体水平上发现了 38 个具有显著意义的 SNP（5%的显著水平）。在 DCC 基因中的 SNP rs402740419 处发现了最显著的关联（P = 1.61×10），该基因在耳朵发育中起着关键作用。此外，我们还观察到另外两个显著的 SNP，PTPRD 中的 rs407891215 和 SOX5 中的 rs407769095，这两个 SNP 都与耳朵发育过程有功能关联。我们的研究结果对未来的绵羊育种有用，并为绵羊和其他家畜耳朵大小发育的遗传基础提供了新的见解。