Probing Cancer Cells through Intracellular Aggregation-Induced Emission Kinetic Rate of Copper Nanoclusters.

pH-responsive luminescent copper nanoclusters (Cu NCs) with aggregation-induced emission (AIE) characteristics have been synthesized. Upon internalization into living cells, the NCs displayed a cellular pH environment-dependent luminescence change with orange-red emission at pH 4.5, whereas bright green emission was observed over time at pH 7.4, through their AIE attributes. Furthermore, the intracellular AIE kinetics of the NC probe was measured in MCF-7 cells and compared to that of HEK-293 cells. Intriguingly, the intracellular rate constant value derived for AIE kinetics in MCF-7 cells was found to be 3-fold higher than that in HEK-293 cell lines, whereas the value was 2-fold higher than that observed in aqueous medium. This provided a new platform to study different cell lines based on intracellular AIE in living cells, with additional potential for future applications in cellular imaging, diagnostics, and disease detection.