National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4, Canada.
National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4, Canada; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No 1, Xujiaping, Lanzhou, Gansu 730046, PR China.
Vaccine. 2018 Jun 18;36(26):3802-3808. doi: 10.1016/j.vaccine.2018.05.045.
Foot-and-mouth disease serotype O viruses (FMDV/O) are responsible for the most outbreaks in FMD endemic countries. O1/BFS is one of the recommended FMD/O vaccine strains by World Reference Laboratory for FMD. In the current study, FMDV/O1 BFS vaccine strain and serotype O field isolates (45) were analyzed phylogenetically and antigenically to gain more insight into the genetic and antigenic characteristics of the vaccine strain and field isolates. O1/BFS showed similarity with 89% of the field isolates using a virus neutralization test (VNT). The P1 region encoding the FMDV capsid was sequenced and analysed for 46 strains of FMDV/O. Phylogenetic analysis showed these viruses originated from five continents and covered eight of 11 reported topotypes. Five isolates that demonstrated low antigenic similarities with O1/BFS were analyzed for their antigenic variation at the known neutralizing antigenic sites. Three of the five isolates demonstrated unique amino acid substitutions at various antigenic sites. No unique amino acid substitutions were observed for the other two unmatched isolates. Positively selected residues were identified on the surface of the FMD virus capsid supporting that it is important to continuously monitor field isolates for their antigenic and phenotypic changes. In conclusion, the vaccine strain O1/BFS is likely to confer protection against 89% of the 45 FMDV/O isolates based on VNT. Thus O1/BFS vaccine strain is still suitable for use in global FMD serotype O outbreak control. Combining data from phylogenetic, molecular and antigenic analysis can provide improvements in the process of vaccine selection.
口蹄疫血清型 O 病毒（FMDV/O）是造成口蹄疫流行国家疫情爆发的主要原因。O1/BFS 是世界口蹄疫参考实验室推荐的 FMD/O 疫苗株之一。在本研究中，对 FMDV/O1 BFS 疫苗株和血清型 O 田间分离株（45 株）进行了系统进化和抗原性分析，以更深入了解疫苗株和田间分离株的遗传和抗原特征。使用病毒中和试验（VNT），O1/BFS 与 89%的田间分离株具有相似性。对 46 株 FMDV/O 的 P1 区编码 FMDV 衣壳进行了测序和分析。系统进化分析表明，这些病毒起源于五个大洲，涵盖了报告的 11 个拓扑型中的 8 个。对与 O1/BFS 抗原性相似性较低的 5 个分离株进行了抗原变异分析，确定了已知中和抗原表位的抗原性变化。5 个分离株在不同的抗原表位显示出独特的氨基酸取代。对于另外两个不匹配的分离株，未观察到独特的氨基酸取代。在 FMD 病毒衣壳的表面鉴定出阳性选择的残基，支持持续监测田间分离株的抗原和表型变化非常重要。总之，根据 VNT，疫苗株 O1/BFS 可能对 45 株 FMDV/O 分离株中的 89%提供保护。因此，O1/BFS 疫苗株仍然适合用于全球 FMD 血清型 O 爆发的控制。将系统进化、分子和抗原分析的数据结合起来，可以提高疫苗选择的过程。
