ICAR-Indian Institute of Agricultural Biotechnology (IIAB), IINRG Campus, Namkum Ranchi, Jharkhand-834010, India.
School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
Chemosphere. 2018 Sep;206:560-567. doi: 10.1016/j.chemosphere.2018.05.018. Epub 2018 May 4.
With the enhancement of commercial manifestation of silver nanoparticles, concerned has risen on their accumulation in aquatic system and consequent effects on fish development and metabolism. In this study, experiments were conducted to assess the impacts of silver nanoparticles on early life cycles of fish considering Zebrafish (Danio rerio) as experimental model. Silver nanoparticles were synthesized through chemical reduction method and characterized through UV-visible spectroscopy, dynamic light scattering (DLS), and HR-TEM. Different sub lethal doses of nanosilver were applied (13.6, 21.6, 42.4, 64, and 128 μgL) to post-fertilization phases of Zebrafish embryos and their interaction effects were monitored up to six days period. No significant morphological variations were observed at 13.6, 21.6, 42.4 μgL dose of silver nanoparticles, whereas 64 and 128 μgL exposure dose exhibited bending in myotome, deformity in tail region, somites, notochord and swelling in anterior and posterior region of embryos and larva. Hatching performances analysis elicited highest hatching success in 13.6 and 21.6 μgL doses of silver nanoparticles followed by positive and negative control, whereas exposure dose of 64 and 128 μgL exhibited comparatively lower success. Western blot analysis were conducted on developing hatchlings with Oct4 antibody and at 13.6 and 21.6 μgLdose,it showed over expression elucidating stimulatory role of nanosilver in these mentioned doses. In silico analysis depicted a firm interaction of nanosilver with Oct4 revealing their key role in growth stimulation of developing embryos. The study demonstrates the function of nanosilver as a growth promoter rather only as a toxicant in fish metabolic system.
随着银纳米粒子商业表现的增强，人们对其在水生系统中的积累以及对鱼类发育和新陈代谢的影响产生了关注。在这项研究中，我们以斑马鱼（Danio rerio）为实验模型，进行了评估银纳米粒子对鱼类早期生命周期影响的实验。通过化学还原法合成银纳米粒子，并通过紫外-可见光谱、动态光散射（DLS）和高分辨率透射电子显微镜（HR-TEM）进行了表征。将不同亚致死剂量的纳米银（13.6、21.6、42.4、64 和 128 µgL）应用于斑马鱼胚胎的后受精阶段，并监测其至六天的相互作用效应。在 13.6、21.6、42.4 µgL 剂量的银纳米粒子下，没有观察到明显的形态变化，而 64 和 128 µgL 的暴露剂量表现出肌节弯曲、尾部区域畸形、体节、脊索和胚胎及幼虫的前后区域肿胀。孵化性能分析表明，在 13.6 和 21.6 µgL 剂量的银纳米粒子下孵化成功率最高，其次是阳性和阴性对照，而 64 和 128 µgL 的暴露剂量则显示出较低的成功率。用 Oct4 抗体对发育中的孵化仔鱼进行了 Western blot 分析，在 13.6 和 21.6 µgL 剂量下，结果显示过度表达，说明纳米银在这些剂量下具有刺激作用。计算机分析表明，纳米银与 Oct4 之间存在牢固的相互作用，揭示了它们在发育中胚胎生长刺激中的关键作用。该研究表明，纳米银在鱼类代谢系统中不仅作为一种毒物，而且还具有作为生长促进剂的功能。
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