College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100193, China; College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Insect Biochem Mol Biol. 2018 Jul;98:34-47. doi: 10.1016/j.ibmb.2018.05.003. Epub 2018 May 17.
Insect general odorant binding proteins (GOBPs) have been long thought to bind and transport host plant volatiles to the olfactory receptors on the dendrite membrane of the olfactory neurons. Recent studies indicate that they can also bind female sex pheromones. In present study, two GOBP genes, AipsGOBP1 and AipsGOBP2 were cloned from the adult antennae of Agrotis ipsilon. Tissue expression profiles indicated that both of them are antennae-specific and more abundant in the female antennae than in the male antennae. Temporal expression profiles showed that both AipsGOBP1 and AipsGOBP2 began to express in antennae 3 days prior to adult emergence from pupae, and reached their highest expression level 3 and 4 days after adult emergence, respectively. Mating increased their expression in the female antennae but reduced their expression in the male antennae. In situ hybridization and immunolocalization demonstrated that both AipsGOBP1 and AipsGOBP2 are expressed and co-localized in sensilla basiconica and sensilla trichodea of both sexes. AipsGOBP2 exhibited a high binding affinity in vitro with the two major sex pheromone components Z7-12:Ac and Z9-14:Ac and the four plant volatiles cis-3-hexen-1-ol, oleic acid, dibutyl phthalate and β-caryophyllene with Ki values less than 5 μM. AipsGOBP1, on the other hand, showed medium binding affinities with the five A. ipsilon sex pheromones and six plant volatiles. AipsGOBP2 also showed a broader ligand-binding spectrum and a greater ligand-binding affinity than AipsGOBP1 with the tested aldehyde and alcohol sex pheromones of Lepidoptera species. Taken together, our results indicate that AipsGOBP2 may play greater roles than AipsGOBP1 does in binding sex pheromones and host plant volatiles.
昆虫气味结合蛋白（GOBP）长期以来一直被认为可以结合和转运植物挥发物到触角感觉神经元树突膜上的嗅觉受体。最近的研究表明，它们也可以结合雌性性信息素。本研究从斜纹夜蛾 Agrotis ipsilon 成虫触角克隆了两个 GOBP 基因，AipsGOBP1 和 AipsGOBP2。组织表达谱表明，它们都是触角特异性的，在雌性触角中比在雄性触角中更为丰富。时间表达谱表明，AipsGOBP1 和 AipsGOBP2 都在成虫从蛹中出现前 3 天开始在触角中表达，并分别在成虫出现后 3 天和 4 天达到最高表达水平。交配增加了雌虫触角中的表达，而减少了雄虫触角中的表达。原位杂交和免疫定位表明，AipsGOBP1 和 AipsGOBP2 在两性的感觉毛和毛感器中都有表达和共定位。AipsGOBP2 在体外与两种主要性信息素成分 Z7-12：Ac 和 Z9-14：Ac 以及四种植物挥发物顺-3-己烯-1-醇、油酸、邻苯二甲酸二丁酯和β-石竹烯具有高结合亲和力，Ki 值均小于 5μM。另一方面，AipsGOBP1 对 5 种斜纹夜蛾性信息素和 6 种植物挥发物表现出中等的结合亲和力。与测试的鳞翅目昆虫的醛和醇性信息素相比，AipsGOBP2 还表现出更广泛的配体结合谱和更高的配体结合亲和力。总的来说，我们的结果表明，AipsGOBP2 在结合性信息素和寄主植物挥发物方面可能比 AipsGOBP1 发挥更大的作用。
