Ishiguro Masanori, Kobayashi Suguru, Matsuyama Kiyoji, Nagamine Takashi
Department of Systems Neuroscience, Sapporo Medical University School of Medicine, South 1, West 17, Chuo-ku, Sapporo, 060-8556, Japan.
Laboratory of Functional Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, South 1, West 17, Chuo-ku, Sapporo, 060-8556, Japan.
Neurosci Res. 2019 Jun;143:13-19. doi: 10.1016/j.neures.2018.05.003. Epub 2018 May 17.
Propofol (2, 6-diisopropylphenol) is one of the most popular intravenous anesthetic agents. In this study, we compared the effects of propofol on inhibitory postsynaptic currents (IPSCs) induced by single and paired electrical stimulations in CA1 pyramidal cells (CA1-PCs) and dentate gyrus granule cells (DG-GCs) in rat hippocampal slices using the whole cell patch-clamp technique. In the absence of propofol, the amplitude of evoked IPSC by single stimulation and decay time constants were stable in both CA1-PCs and DG-GCs for 30 min. Propofol (1 μM and 10 μM) increased both IPSC amplitude in CA1-PCs, but not in DG-GCs. Further, using a paired pulse stimulation protocol, the ratio of IPSC amplitudes (the second response: A2/the first response: A1) was increased by propofol in CA1, but not in DG-GCs. These results suggest that propofol selectively affects IPSCs in CA1-PCs, which is similar to previously reported actions of benzodiazepines.
丙泊酚（2,6 - 二异丙基苯酚）是最常用的静脉麻醉剂之一。在本研究中，我们使用全细胞膜片钳技术，比较了丙泊酚对大鼠海马脑片CA1锥体神经元（CA1-PCs）和齿状回颗粒细胞（DG-GCs）中单次和配对电刺激诱发的抑制性突触后电流（IPSCs）的影响。在没有丙泊酚的情况下，CA1-PCs和DG-GCs中单次刺激诱发的IPSC幅度和衰减时间常数在30分钟内保持稳定。丙泊酚（1μM和10μM）增加了CA1-PCs中的IPSC幅度，但对DG-GCs没有影响。此外，使用配对脉冲刺激方案，丙泊酚增加了CA1中IPSC幅度的比值（第二个反应：A2/第一个反应：A1），但对DG-GCs没有影响。这些结果表明，丙泊酚选择性地影响CA1-PCs中的IPSCs，这与先前报道的苯二氮䓬类药物的作用相似。
