Hauptfeld M, Hauptfeld V, Zeff R, Shreffler D C
Department of Genetics, Washington University, School of Medicine, St. Louis, MO 63310.
J Mol Cell Immunol. 1988;4(1):1-8.
Four new intra-H-2 recombinants were characterized serologically and functionally. In two of these recombinants, B10.ASR1 and B10.ASR7, crossing over occurred between the A alpha and E alpha subregions, very probably in E beta since most intra-I region recombinants thus far investigated at the DNA level appear to involve recombination within the E beta gene. In the other two recombinants, B10.ASR2 and B10.ARS8, crossing over occurred between the S and D subregions. B10.ASR2 and B10.ASR8, crossing over occurred between the S and D subregions. B10.ASR7, which is serologically indistinguishable from B10.BASR1 and B10.S(8R), slightly stimulates and strongly responds to both of these strains in MLR. The probable location of the B10.S(8R) stimulatory product is E beta. The H-2 composition of B10.ASR1 is closest to that of B10.S(9R) and B10.HTT; therefore, precise definition of the cross-over point at the DNA level will be of particular interest.
对四个新的H-2复合体内重组体进行了血清学和功能特征分析。在其中两个重组体B10.ASR1和B10.ASR7中，交换发生在Aα和Eα亚区之间，很可能发生在Eβ区，因为迄今为止在DNA水平上研究的大多数I区内重组体似乎都涉及Eβ基因内的重组。在另外两个重组体B10.ASR2和B10.ARS8中，交换发生在S和D亚区之间。B10.ASR7在血清学上与B10.BASR1和B10.S(8R)无法区分，在混合淋巴细胞反应（MLR）中对这两种菌株有轻微刺激并强烈反应。B10.S(8R)刺激产物的可能位置是Eβ区。B10.ASR1的H-2组成最接近B10.S(9R)和B10.HTT；因此，在DNA水平上精确确定交换点将特别有意义。
