Rashid Hina, Sharma Shikha, Beigh Saba, Ahmad Firoz, Raisuddin Sheikh
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi - 110062, India.
Centre of Research for Development (CORD), The University of Kashmir, Srinagar - 190006, India.
Endocr Metab Immune Disord Drug Targets. 2018;18(6):626-636. doi: 10.2174/1871530318666180521095443.
Bisphenol A (BPA) is suspected to cause hormonal imbalance in humans. Dietary factors are known to bring changes in hormonal profile. In order to study chemico-biological interaction of iron deficiency on toxicity outcome of BPA exposure, we studied the modulatory effects of iron deficiency on the hormone levels in rats chronically-exposed to BPA.
Weanling rats maintained on normal and iron-deficient diets were exposed to low level of BPA at 0, 1, 5 and 10 ppm for six months through drinking water. The serum levels of thyroidstimulating hormone (TSH), testosterone, progesterone and estradiol were measured in the animals by enzyme-linked immunosorbent assay kit. Histopathology was performed to check the pathological changes in gonads.
No significant change was observed in TSH, progesterone and estradiol levels at 1 and 5 ppm BPA. However, at 10 ppm BPA a significant increase in TSH level was observed in the animals maintained on an iron-deficient diet of either sex. BPA caused a significant change in testosterone level even at 5 and 10 ppm doses in animals of either sex. However, in male rats 1 ppm dose also showed a significant effect in the animals maintained on iron deficient diet. Changes in the histoarchitecture of the testes at high dose of BPA (10 ppm) were more remarkable in anemic rats.
These results suggest that iron deficiency has no generalized effect on hormonal levels in BPA-treated animals and trends indicate a more remarkable effect in male animals at hormonal and tissue levels.
双酚A（BPA）被怀疑会导致人体激素失衡。已知饮食因素会引起激素水平的变化。为了研究缺铁对BPA暴露毒性结果的化学-生物学相互作用，我们研究了缺铁对长期暴露于BPA的大鼠激素水平的调节作用。
将断奶大鼠分别饲养在正常饮食和缺铁饮食中，通过饮用水使其暴露于0、1、5和10 ppm的低水平BPA中六个月。用酶联免疫吸附测定试剂盒测量动物血清中促甲状腺激素（TSH）、睾酮、孕酮和雌二醇的水平。进行组织病理学检查以查看性腺的病理变化。
在1和5 ppm BPA时，TSH、孕酮和雌二醇水平未观察到显著变化。然而，在10 ppm BPA时，无论雌雄，饲养在缺铁饮食中的动物TSH水平均显著升高。即使在5和10 ppm剂量下，BPA也会导致雌雄动物的睾酮水平发生显著变化。然而，在雄性大鼠中，1 ppm剂量对饲养在缺铁饮食中的动物也显示出显著影响。高剂量BPA（10 ppm）时，贫血大鼠睾丸的组织结构变化更为明显。
这些结果表明，缺铁对BPA处理动物的激素水平没有普遍影响，趋势表明在激素和组织水平上对雄性动物的影响更为显著。
