[视紫红质在无细胞翻译系统中的合成。I. 合成的牛视蛋白mRNA结构对其翻译效率的影响]
[Synthesis of visual rhodopsin in a cell-free translation system. I. Influence of the structure of the synthetic bovine visual opsin mRNA on its translational efficiency].
作者信息
Zozulia S A, Gurevich V V, Shmukler B E, Namochin M Iu, Zviaga T A
出版信息
Bioorg Khim. 1988 Dec;14(12):1663-70.
PMID:2978006
Abstract
Influence of structural changes in nontranslated regions and translation initiation site of the in vitro synthesized bovine opsin mRNA on its translational efficiency in the wheat germ cell-free system has been studied. It is shown that level of the opsin synthesis up to 30 micrograms per 1 ml of translational mixture can be attained by optimizing structure of 5'-nontranslated region.
摘要
研究了体外合成的牛视蛋白mRNA的非翻译区和翻译起始位点的结构变化对其在小麦胚无细胞体系中翻译效率的影响。结果表明，通过优化5'-非翻译区的结构，每1毫升翻译混合物中视蛋白的合成水平可达30微克。
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引用本文的文献
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In vitro synthesis of visual rhodopsin for a protein engineering study.
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