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Comprehensive Quantitation Using Two Stable Isotopically Labeled Species and Direct Detection of N-Acyl Moiety of Sphingomyelin.使用两种稳定同位素标记物种进行综合定量并直接检测鞘磷脂的N-酰基部分
Lipids. 2017 Sep;52(9):789-799. doi: 10.1007/s11745-017-4279-5. Epub 2017 Aug 2.
2
Detailed Structural Characterization of Sphingolipids via 193 nm Ultraviolet Photodissociation and Ultra High Resolution Tandem Mass Spectrometry.通过 193nm 紫外光光解和超高分辨串联质谱对神经酰胺进行详细的结构表征。
J Am Soc Mass Spectrom. 2017 Jul;28(7):1406-1419. doi: 10.1007/s13361-017-1668-1. Epub 2017 Apr 28.
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Synthesis and degradation pathways, functions, and pathology of ceramides and epidermal acylceramides.神经酰胺和表皮酰基神经酰胺的合成和降解途径、功能及病理学。
Prog Lipid Res. 2016 Jul;63:50-69. doi: 10.1016/j.plipres.2016.04.001. Epub 2016 Apr 21.
4
In-depth sphingomyelin characterization using electron impact excitation of ions from organics and mass spectrometry.利用有机物离子的电子轰击激发和质谱法对鞘磷脂进行深入表征。
J Lipid Res. 2016 May;57(5):858-67. doi: 10.1194/jlr.M067199. Epub 2016 Mar 22.
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Lysophospholipid receptors in drug discovery.药物研发中的溶血磷脂受体
Exp Cell Res. 2015 May 1;333(2):171-177. doi: 10.1016/j.yexcr.2014.11.020. Epub 2014 Dec 8.
6
Selecting the correct weighting factors for linear and quadratic calibration curves with least-squares regression algorithm in bioanalytical LC-MS/MS assays and impacts of using incorrect weighting factors on curve stability, data quality, and assay performance.在生物分析液相色谱-串联质谱（LC-MS/MS）分析中，使用最小二乘法回归算法为线性和二次校准曲线选择正确的加权因子，以及使用不正确的加权因子对曲线稳定性、数据质量和分析性能的影响。
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Differentiation of complex lipid isomers by radical-directed dissociation mass spectrometry.自由基定向解离质谱法对复杂脂质异构体的区分。
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Ozone-induced dissociation: elucidation of double bond position within mass-selected lipid ions.臭氧诱导解离：质谱选择脂质离子中双键位置的阐明
Anal Chem. 2008 Jan 1;80(1):303-11. doi: 10.1021/ac7017684. Epub 2007 Dec 7.
9
Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry.鞘脂组学：通过液相色谱串联质谱法对鞘脂进行高通量、结构特异性和定量分析。
Methods. 2005 Jun;36(2):207-24. doi: 10.1016/j.ymeth.2005.01.009.
10
Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3.通过条件性中性丢失扫描和MS3对磷脂酰胆碱和鞘磷脂中的分子种类进行快速和选择性鉴定。
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使用两种稳定同位素标记的鞘磷脂物种通过液相色谱-质谱联用法定量和定性分析鞘磷脂的方法

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species.

作者信息

Hama Kotaro, Fujiwara Yuko, Yokoyama Kazuaki

机构信息

Faculty of Pharmaceutical Sciences, Teikyo University;

Faculty of Pharmaceutical Sciences, Teikyo University.

出版信息

J Vis Exp. 2018 May 7(135):57293. doi: 10.3791/57293.

DOI:10.3791/57293
PMID:29782002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6101123/
Abstract

We present a method of analyzing sphingomyelin (SM) qualitatively and quantitatively by liquid chromatography-electrospray Ionization-tandem mass spectrometry (LC-ESI-MS/MS). SM is a common sphingolipid composed of a phosphorylcholine and a ceramide as the hydrophilic and hydrophobic component, respectively. A number of SM species are present in mammalian cells due to a variety in the sphingoid long chain base (LCB) and an N-acyl moiety in the ceramide. In this report, we show a method of estimating the number of carbon and double bonds in a LCB and an N-acyl moiety based on their corresponding product ions in MS/MS/MS (MS) experiments. In addition, we present a quantitative analysis method for SM using two stable isotopically labeled SM species, which facilitates determining the range used in SM quantitation. The present method will be useful in characterizing a variety of SM species in biological samples and industrial products such as cosmetics.

摘要

我们提出了一种通过液相色谱-电喷雾电离串联质谱法（LC-ESI-MS/MS）对鞘磷脂（SM）进行定性和定量分析的方法。SM是一种常见的鞘脂，分别由磷酸胆碱和神经酰胺作为亲水和疏水成分组成。由于鞘氨醇长链碱基（LCB）和神经酰胺中的N-酰基部分存在多种变化，哺乳动物细胞中存在多种SM种类。在本报告中，我们展示了一种基于MS/MS/MS（MS）实验中相应的产物离子来估计LCB和N-酰基部分中碳和双键数量的方法。此外，我们提出了一种使用两种稳定同位素标记的SM种类对SM进行定量分析的方法，这有助于确定SM定量中使用的范围。本方法将有助于表征生物样品和化妆品等工业产品中的各种SM种类。