Chasalow F I, Blethen S L, Bradlow H L
Division of Pediatric Endocrinology, Schneider Children's Hospital of Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Steroids. 1988 Sep;52(3):205-15. doi: 10.1016/0039-128x(88)90004-9.
We assayed Type 1 (high K+) and Type 2 (high Na+) human breast cyst fluids for DHEA-S. When an antibody specific for the 3-sulfoconjugate end of DHEA-S was used, Type 1 cyst fluids (n = 18) showed a content of 114 +/- 68 micrograms/mL (mean +/- sigma) and Type 2 cyst fluids (n = 14) of 35 +/- 17 micrograms/mL (P less than 0.01). Using an antibody specific for the D-ring, the results were 151 +/- 91 micrograms/mL and 51 +/- 32 micrograms/mL, respectively (P less than 0.01). The apparent concentrations of DHEA-S were statistically different, even though both assays gave equal results in serum from normal adults. The presence of other compounds in individual cyst fluid samples was examined by extraction and chromatography. DHEA-S immunoreactivity was found in both early and late eluting fractions in Type 1 cyst fluids and in late eluting fractions from Type 2 cyst fluids. Only the late eluting fraction from Type 2 fluids had approximately equal immunoreactivity with both antibodies. In addition to authentic DHEA-S, breast cyst fluids contain other materials that react with DHEA-S antibodies. Radioimmunoassays for DHEA-S in cyst fluid must be specifically validated because of the presence of these compounds.
我们检测了1型（高钾）和2型（高钠）人乳腺囊肿液中的硫酸脱氢表雄酮（DHEA-S）。当使用针对DHEA-S 3-硫酸共轭末端的特异性抗体时，1型囊肿液（n = 18）的含量为114±68微克/毫升（平均值±标准差），2型囊肿液（n = 14）的含量为35±17微克/毫升（P<0.01）。使用针对D环的特异性抗体时，结果分别为151±91微克/毫升和51±32微克/毫升（P<0.01）。尽管两种检测方法在正常成年人血清中给出的结果相同，但DHEA-S的表观浓度在统计学上存在差异。通过萃取和色谱法检测了各个囊肿液样本中其他化合物的存在情况。在1型囊肿液的早期和晚期洗脱组分中均发现了DHEA-S免疫反应性，在2型囊肿液的晚期洗脱组分中也发现了免疫反应性。只有2型囊肿液的晚期洗脱组分与两种抗体的免疫反应性大致相同。除了真正的DHEA-S外，乳腺囊肿液还含有其他与DHEA-S抗体发生反应的物质。由于这些化合物的存在，囊肿液中DHEA-S的放射免疫测定必须进行专门验证。
