基于铂镍纳米立方体-L-半胱氨酸-鲁米诺纳米复合材料的超灵敏电化学发光免疫传感器。
An ultrasensitive electrochemiluminescence immunosensor based on platinum nickel nanocubes-L-cysteine-luminol nanocomposite.
机构信息
The Education Ministry Key Laboratory of Resource Chemistry Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
The Education Ministry Key Laboratory of Resource Chemistry Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
出版信息
Talanta. 2018 Aug 15;186:322-329. doi: 10.1016/j.talanta.2018.04.069. Epub 2018 Apr 26.
An ultrasensitive electrochemiluminescence (ECL) immunosensor was initially developed for quantitative detection of carbohydrate antigen 15-3 (CA15-3) using platinum nickel nanocubes-L-cysteine-luminol nanocomposite (PtNi NCs-L-Cys-luminol) as signal probe. Herein, the PtNi NCs-L-Cys-luminol nanocomposite was modified on the glassy carbon electrode (GCE) surface via the film-forming properties of chitosan. Then, the CA15-3 antibody was attached to the modified electrode surface by amidating reaction to construct the ECL immunosensor. Experimental results showed that with the capturing of CA15-3 antigen molecules on the immunosensor, the ECL signal intensity observably decreased, indicating the quenching detection principle of electrochemiluminescence. Under optimal experimental conditions, the constructed ECL immunosensor displayed remarkable performance for CA15-3 detection ranging from 0.0005 U/mL to 500 U/mL, with a relatively low detection limit of 0.000167 U/mL (S/N = 3). It might be ascribed to the fact that the PtNi NCs could dramatically promote the decomposition of HO to produce various active free radical, thereby the ECL responses of luminol were prominently magnified and its sensitivity was effectively increased. The immunosensor exemplified its advantages of easy fabrication, fast analysis, high sensitivity, good reproducibility and selectivity. Moreover, the recovery tests exhibited that the sensor can be used to sensitively detect CA15-3 in serum samples, suggesting potential application prospect in bioanalysis.
一种超灵敏的电化学发光（ECL）免疫传感器最初被开发出来，用于使用铂镍纳米立方体-L-半胱氨酸-鲁米诺纳米复合材料（PtNi NCs-L-Cys-luminol）作为信号探针定量检测糖抗原 15-3（CA15-3）。在此，通过壳聚糖的成膜特性，将 PtNi NCs-L-Cys-luminol 纳米复合材料修饰在玻碳电极（GCE）表面。然后，通过酰胺化反应将 CA15-3 抗体附着在修饰电极表面上，以构建 ECL 免疫传感器。实验结果表明，随着免疫传感器上 CA15-3 抗原分子的捕获，ECL 信号强度明显降低，表明了电化学发光的猝灭检测原理。在最佳实验条件下，所构建的 ECL 免疫传感器对 CA15-3 的检测范围从 0.0005 U/mL 到 500 U/mL，检测限相对较低为 0.000167 U/mL（S/N = 3）。这可能是因为 PtNi NCs 可以极大地促进 HO 的分解，从而产生各种活性自由基，从而显著放大了鲁米诺的 ECL 响应，并有效提高了其灵敏度。该免疫传感器例证了其易于制造、快速分析、高灵敏度、良好重现性和选择性的优点。此外，回收测试表明，该传感器可用于灵敏检测血清样本中的 CA15-3，表明其在生物分析中具有潜在的应用前景。
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