基于17个微卫星标记的波斯尼亚和黑塞哥维那纯血马种群的遗传多样性

Genetic diversity of Thoroughbred horse population from Bosnia and Herzegovina based on 17 microsatellite markers.

作者信息

Rukavian Dunja, Hasanbasic Danica, Ramic Jasmin, Zahirovic Amir, Ajanovic Atifa, Beganovic Kemal, Durmic-Pasic Adaleta, Kalamujic Belma, Pojskic Naris

出版信息

Jpn J Vet Res. 2016 Sep;64(3):215-220.

PMID:29786993

Abstract

The focus of this study was on genetic diversity of TB horse population raised in B&H. Genomic DNA was genotyped by using 17 microsatellite markers. A total of 103 alleles were detected. The average number of alleles per locus was 6.059 and effective number of alleles was 3.293. Means of observed and expected heterozygosity were calculated 0.645 and 0.696, respectively. The average PIC values was 0.649 and inbreeding coefficient was 0.090. Based on all observed parameters, ASB2 locus showed the highest genetic diversity while locus HMS2 was the least diverse. These results suggest that the population of TB horses from B&H is not affected by substantial loss of genetic diversity, indicating the presence of reasonably high level of genetic variability.

摘要

本研究的重点是波斯尼亚和黑塞哥维那饲养的纯血马种群的遗传多样性。使用17个微卫星标记对基因组DNA进行基因分型。共检测到103个等位基因。每个位点的平均等位基因数为6.059，有效等位基因数为3.293。观察到的杂合度和预期杂合度的均值分别计算为0.645和0.696。平均多态信息含量值为0.649，近交系数为0.090。基于所有观察到的参数，ASB2位点显示出最高的遗传多样性，而HMS2位点的多样性最低。这些结果表明，波斯尼亚和黑塞哥维那的纯血马种群不受遗传多样性大量丧失的影响，表明存在相当高水平的遗传变异性。