Hayashi Yohei, Matsui Yasuhisa
Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai, Miyagi, Japan.
Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.
Methods Mol Biol. 2019;2045:259-269. doi: 10.1007/7651_2018_164.
Primordial germ cells (PGCs), the precursors of gametes, are the only cells capable of acquiring totipotency upon fertilization, but the molecular mechanisms regulating germ cell characteristics have not been fully elucidated. Although intracellular metabolic status and regulation are responsible for the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.
原始生殖细胞（PGCs）是配子的前体，是唯一在受精时能够获得全能性的细胞，但调节生殖细胞特征的分子机制尚未完全阐明。尽管细胞内代谢状态和调节负责细胞功能和分化的控制，但对PGCs的代谢特征知之甚少。在这里，我们描述了使用一种综合的代谢组学、蛋白质组学和能量代谢分析方法，通过质谱法全面阐明PGCs的代谢特征。
