Marine and Coastal Research Institute "José Benito Vives de Andréis"- INVEMAR, Santa Marta D.T.C.H., Colombia.
Korea Institute of Ocean Science & Technology, Marine Biotechnology Research, Ansan, Korea.
J Appl Microbiol. 2018 Sep;125(3):753-765. doi: 10.1111/jam.13926. Epub 2018 Jun 29.
To isolate micro-organisms associated with marine coastal sediments of Colombian Caribbean Sea and for evaluating its antimicrobial activity in order to identify the most active strains.
One hundred and four strains were isolated from sediment samples of the Colombian Caribbean Sea. First at all, an antimicrobial activity screening was made using agar well diffusion method against the pathogens: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans, Candida tropicalis and Pseudomonas aeruginosa. Seventeen strains showed strong antimicrobial activity and were identified as members of the Streptomyces, Micrococcus and Bacillus genera. Organic extracts were produced by liquid-liquid extraction and HPLC profiles of the most active extracts were obtained. Then, the antimicrobial activity of the extracts was evaluated with the broth microdilution test, finding antimicrobial activities superior to 90% against S. aureus MRSA and C. albicans. HPLC profiles indicated the presence of different antimicrobial compounds.
This study demonstrates that the microorganisms isolated from the Colombian Caribbean Sea are possible sources of antimicrobial compounds against pathogenic strains.
These results contribute to the knowledge of the biotechnological potential of the Colombian biodiversity for the development of pharmaceutical products that can counteract the increasing problem of pathogen resistance.
从哥伦比亚加勒比海沿海沉积物中分离与海洋有关的微生物，并评估其抗菌活性，以鉴定最具活性的菌株。
从哥伦比亚加勒比海的沉积物样本中分离出 104 株菌株。首先，采用琼脂孔扩散法对病原菌：大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、白色念珠菌、热带假丝酵母和铜绿假单胞菌进行了抗菌活性筛选。17 株表现出较强的抗菌活性，被鉴定为链霉菌属、微球菌属和芽孢杆菌属的成员。通过液液萃取法制备了有机提取物，并获得了最活跃提取物的 HPLC 图谱。然后，通过肉汤微量稀释试验评估了提取物的抗菌活性，发现对金黄色葡萄球菌 MRSA 和白色念珠菌的抗菌活性超过 90%。HPLC 图谱表明存在不同的抗菌化合物。
本研究表明，从哥伦比亚加勒比海分离的微生物可能是对抗致病菌株的抗菌化合物的来源。
这些结果有助于了解哥伦比亚生物多样性的生物技术潜力，可用于开发能对抗病原菌耐药性日益增加问题的药物产品。
