1 Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, U.S.A.
2 School of Biological Sciences, Washington State University; and.
Mol Plant Microbe Interact. 2018 Nov;31(11):1117-1120. doi: 10.1094/MPMI-04-18-0107-A. Epub 2018 Sep 7.
Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.
条锈菌引起小麦条锈病，禾柄锈菌引起大麦条锈病。有几个条锈菌小麦专化型基因组，但禾柄锈菌的基因组尚未公布。为了了解条锈菌小麦专化型和禾柄锈菌的基因组进化和致病性的分子机制，需要更多的条锈菌小麦专化型和禾柄锈菌基因组。我们使用 PacBio 和 Illumina 技术以及 RNA 测序对条锈菌小麦专化型 93-210 分离株和禾柄锈菌 93TX-2 分离株进行了测序。它们的基因组序列被组装成具有高连续性的 contigs，并显示出显著的结构差异。两个菌株的圆形线粒体基因组都是完整的。这些基因组为破译快速进化和宿主适应性的基因组基础、鉴定无毒基因和其他重要性状以及研究宿主-病原体相互作用提供了高质量的资源。
