Punjabi U, Van Mulders H, Goovaerts I, Peeters K, Clasen K, Janssens P, Zemtsova O, De Neubourg D
Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Clin Biochem. 2018 Dec;62:47-54. doi: 10.1016/j.clinbiochem.2018.05.011. Epub 2018 May 21.
Sperm DNA fragmentation measured by different techniques make comparisons impossible due to lack of standardization. Induction of DNA damage after sperm preparation in the entire fraction has been observed on independent occasions but findings are not consistent.
Men presenting at a University hospital setup for infertility treatment. DNA damage via TUNEL assay was validated on fresh semen samples, as conventional semen parameters, to reduce variability of results.
Sperm motility in neat semen inversely correlated with sperm DNA fragmentation in the total fraction, but, total count, leukocytes and immature germ cells significantly affected the vital fraction. Sperm DNA fragmentation was observed both in normal and subnormal semen samples, but was significantly different in the total fraction of astheno-, asthenoterato- and oligoteratozoospermic men. After density gradient centrifugation, sperm DNA fragmentation increased significantly in the total but decreased in the vital fraction. Advancing male age significantly influenced damage in the total but not in the vital population.
These findings provide opportunities to investigate the significance of the total and the vital fractions both in natural conception and after different assisted reproductive technologies.
由于缺乏标准化，通过不同技术测量的精子DNA片段化使得比较变得不可能。在独立的情况下，已观察到在整个部分进行精子制备后DNA损伤的诱导，但结果并不一致。
在大学医院不育治疗机构就诊的男性。通过TUNEL分析对新鲜精液样本中的DNA损伤进行验证，作为常规精液参数，以减少结果的变异性。
纯精液中的精子活力与整个部分的精子DNA片段化呈负相关，但总计数、白细胞和未成熟生殖细胞对有活力部分有显著影响。在正常和亚正常精液样本中均观察到精子DNA片段化，但在弱精子症、弱畸精子症和少畸精子症男性的整个部分中差异显著。密度梯度离心后，整个部分的精子DNA片段化显著增加，而有活力部分则减少。男性年龄的增长对整个部分的损伤有显著影响，但对有活力部分没有影响。
这些发现为研究整个部分和有活力部分在自然受孕以及不同辅助生殖技术后的意义提供了机会。
