Stouthamer Corinne M, Kelly Suzanne, Hunter Martha S
Graduate Interdisciplinary Program in Entomology and Insect Science, PO Box 210036, The University of Arizona, Tucson, AZ 85721, USA; Department of Entomology, 410 Forbes Building, The University of Arizona, Tucson, AZ 85721, USA.
Department of Entomology, 410 Forbes Building, The University of Arizona, Tucson, AZ 85721, USA.
J Microbiol Methods. 2018 Aug;151:16-19. doi: 10.1016/j.mimet.2018.05.013. Epub 2018 May 21.
Symbioses between bacteria and insects are often associated with changes in important biological traits that can significantly affect host fitness. To a large extent, studies of these interactions have been based on physiological changes or induced phenotypes in the host, and the genetic mechanisms by which symbionts interact with their hosts have only recently become better understood. Learning about symbionts has been challenging in part due to difficulties such as obtaining enough high quality genomic material for high throughput sequencing technology, especially for symbionts present in low titers, and in small or difficult to rear non-model hosts. Here we introduce a new method that substantially increases the yield of bacterial DNA in minute arthropod hosts, and requires less starting material relative to previous published methods.
细菌与昆虫之间的共生关系通常与重要生物学特性的变化相关联，这些变化会显著影响宿主的适应性。在很大程度上，对这些相互作用的研究一直基于宿主的生理变化或诱导表型，而共生体与宿主相互作用的遗传机制直到最近才得到更好的理解。了解共生体颇具挑战性，部分原因在于存在一些困难，比如难以获得足够高质量的基因组材料用于高通量测序技术，尤其是对于低滴度存在于小型或难以饲养的非模式宿主中的共生体。在此，我们介绍一种新方法，该方法可大幅提高微小节肢动物宿主中细菌DNA的产量，并且相对于先前发表的方法需要更少的起始材料。
