基于 GR-5 DNAzyme 的氧化石墨烯辅助金纳米粒子条带生物传感器用于快速铅离子检测。
Graphene oxide-assisted Au nanoparticle strip biosensor based on GR-5 DNAzyme for rapid lead ion detection.
机构信息
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Bioinformatics & Molecular Imaging Key Laboratory, Key Laboratory of Biomedical Photonics of Ministry of Education, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Bioinformatics & Molecular Imaging Key Laboratory, Key Laboratory of Biomedical Photonics of Ministry of Education, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
出版信息
Colloids Surf B Biointerfaces. 2018 Sep 1;169:305-312. doi: 10.1016/j.colsurfb.2018.05.020. Epub 2018 May 21.
This study has reported that a GR-5 DNAzyme based lead ion strip biosensor could exhibit an enhanced specificity with the assistance of graphene oxide (GO). This enhancement results from the specific π-stacking interaction between the ribose rings of the nucleobases and the carbon hexagons in GO which can reduce the false positive interference by removing unhybridized ssDNA during the annealing of GR-5 DNAzyme. Meanwhile, conjugate pad was sprayed with two kinds of AuNP-DNA probes, and nitrocellulose membrane test zone and control zone were immobilized with two kinds of biotin-DNA probes, respectively. The limit of detection of this strip biosensor was estimated to be about 0.05 nM (S/N = 3) and 1 nM (with naked eyes) with a linear range from 0.01 to 100 μM. Furthermore, the strip biosensor exhibited excellent selectivity toward Pb in the presence of other divalent metal ions. For real soil samples, the obtained recoveries were in the range from 91.5% to 113.1%.
本研究报道了一种基于 GR-5 DNA 酶的 lead ion 条带生物传感器，在氧化石墨烯（GO）的辅助下可以表现出增强的特异性。这种增强是由于碱基的核糖环与 GO 中的碳六边形之间的特定π堆积相互作用，这可以通过在 GR-5 DNA 酶退火过程中去除未杂交的 ssDNA 来减少假阳性干扰。同时，在结合垫上喷涂了两种 AuNP-DNA 探针，硝酸纤维素膜测试区和对照区分别固定了两种生物素-DNA 探针。该条带生物传感器的检测限估计约为 0.05 nM（S/N = 3）和 1 nM（肉眼可见），线性范围从 0.01 到 100 μM。此外，该条带生物传感器在存在其他二价金属离子的情况下对 Pb 表现出优异的选择性。对于实际土壤样品，获得的回收率在 91.5%至 113.1%之间。
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