Beijing, People's Republic of China.
From the Department of Plastic and Reconstructive Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College.
Plast Reconstr Surg. 2018 Jun;141(6):1395-1407. doi: 10.1097/PRS.0000000000004386.
The authors aimed to analyze factors related to lipotransfer for localized scleroderma, and to explore the feasibility of cell-assisted lipotransfer for localized scleroderma treatment.
Abdominal fat samples were taken from six scleroderma patients without corticosteroid therapy, five scleroderma patients with corticosteroid therapy, and 10 normal liposuction patients. Their quantity, morphology, and proliferation ability were measured. Blood flow was measured by laser speckle contrast imaging in localized scleroderma lesions and normal contralateral regions for eight localized scleroderma patients. Bleomycin-induced skin fibrosis nude mice were also used to investigate differences between lipotransfer and cell-assisted lipotransfer. Fat weight was measured, and expression of transforming growth factor (TGF)-β1 and type III collagen in the injected skin was determined by immunohistochemistry.
The number of stem cells from scleroderma patients with corticosteroid treatment was significantly reduced. Mean blood perfusion in localized scleroderma lesions was not significantly different than in the contralateral normal regions. In normal nude mice, there were no significant changes in TGF-β1 and type III collagen between the control, lipotransfer, and cell-assisted lipotransfer groups, whereas in bleomycin-induced skin fibrosis nude mice, lipotransfer and cell-assisted lipotransfer reduced TGF-β1 and type III collagen expression.
For scleroderma patients, fewer adipose-derived stem cells, because of a history of corticosteroid therapy and a local inflammatory microenvironment, are more important factors, whereas blood supply showed no significant change. Therefore, cell-assisted lipotransfer not only improves the survival rate of transplanted fat but also improves skin texture in bleomycin-induced skin fibrosis nude mice.
作者旨在分析与局部硬皮病脂肪转移相关的因素，并探讨细胞辅助脂肪转移治疗局部硬皮病的可行性。
从未接受皮质类固醇治疗的 6 例硬皮病患者、接受皮质类固醇治疗的 5 例硬皮病患者和 10 例正常吸脂患者中获取腹部脂肪样本。测量其数量、形态和增殖能力。使用激光散斑对比成像技术测量 8 例局部硬皮病患者的局部硬皮病病变和正常对侧区域的血流。还使用博来霉素诱导的皮肤纤维化裸鼠来研究脂肪转移和细胞辅助脂肪转移之间的差异。测量脂肪重量，并通过免疫组织化学法测定注射皮肤中转化生长因子 (TGF)-β1 和 III 型胶原的表达。
接受皮质类固醇治疗的硬皮病患者的干细胞数量明显减少。局部硬皮病病变的平均血流与对侧正常区域无显著差异。在正常裸鼠中，对照组、脂肪转移组和细胞辅助脂肪转移组之间 TGF-β1 和 III 型胶原无显著变化，而在博来霉素诱导的皮肤纤维化裸鼠中，脂肪转移和细胞辅助脂肪转移降低了 TGF-β1 和 III 型胶原的表达。
对于硬皮病患者，由于皮质类固醇治疗史和局部炎症微环境，较少的脂肪来源干细胞是更重要的因素，而血液供应没有明显变化。因此，细胞辅助脂肪转移不仅提高了移植脂肪的存活率，而且改善了博来霉素诱导的皮肤纤维化裸鼠的皮肤质地。
