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[一种基于用彩色硅酮注射血管制造人脑解剖标本的技术(技术说明)]

[A technique of manufacturing anatomical preparations of the human brain based on injecting vessels with colored silicone (a technical note)].

作者信息

Shkarubo M A, Dobrovol'skiy G F, Polev G A, Shkarubo A N, Tarkhnishvili G S, Spitsyna L I, Karnaukhov V V, Bykanov A E

机构信息

Burdenko Neurosurgical Institute, 4-ya Tverskaya-Yamskaya Str., 16, Moscow, Russia, 125047.

Rogachev National Research Center of Pediatric Hematology, Oncology, and Immunology, Moscow, Russia.

出版信息

Zh Vopr Neirokhir Im N N Burdenko. 2018;82(2):59-64. doi: 10.17116/oftalma201882259-64.

DOI:10.17116/oftalma201882259-64
PMID:29795087
Abstract

OBJECTIVE

The objective was to produce anatomical preparations by injecting vessels with colored silicone to study the brain and skull base anatomy.

MATERIAL AND METHODS

Fresh, undissected, and unfixed cadavers were used. The internal carotid arteries and internal jugular veins were identified on both sides of the neck. The vessels were washed with running water. Then, a complex solution consisting of white silicone rubber, silicone oil (solvent), and a coloring pigment (red and blue pigments) at a ratio of 1:(0.9-1.1):(0.04-0.06), respectively, was prepared. About 30-60 s before injecting the complex solution into the vessels, a catalyst-hardener was added to the solution at a ratio of 1:(0.05-0.07). The complex solution was first introduced into the internal carotid artery until the solution came out from the contralateral internal carotid artery; then, the solution was injected into the internal jugular vein until the solution emerged from the contralateral internal jugular vein.

RESULTS

The technique enables quick and high-quality visualization of both large and very small vessels of the brain and skull base.

CONCLUSION

The proposed simple and inexpensive technique of manufacturing anatomical preparations improves the quality of training and mastering of microsurgical skills in residents and practicing neurosurgeons.

摘要

目的

通过向血管内注射彩色硅酮来制作解剖标本,以研究脑和颅底的解剖结构。

材料与方法

使用新鲜、未解剖且未固定的尸体。在颈部两侧识别出颈内动脉和颈内静脉。用流水冲洗血管。然后,制备一种由白色硅橡胶、硅油(溶剂)和着色颜料(红色和蓝色颜料)按1:(0.9 - 1.1):(0.04 - 0.06)的比例组成的复合溶液。在将复合溶液注入血管前约30 - 60秒,按1:(0.05 - 0.07)的比例向溶液中加入催化剂 - 硬化剂。首先将复合溶液引入颈内动脉,直到溶液从对侧颈内动脉流出;然后,将溶液注入颈内静脉,直到溶液从对侧颈内静脉流出。

结果

该技术能够快速且高质量地显示脑和颅底的大血管和非常小的血管。

结论

所提出的制作解剖标本的简单且廉价的技术提高了住院医师和执业神经外科医生在显微外科技能培训和掌握方面的质量。

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