Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo, Zhejiang, P. R. China.
Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing, P. R. China.
PLoS One. 2018 May 24;13(5):e0196231. doi: 10.1371/journal.pone.0196231. eCollection 2018.
The adhesion ability of Lactobacillus plantarum affects retention time in the human gastro-intestinal tract, as well as influencing the interaction with their host. In this study, the relationship between the adhesion activity of, and metabolic changes in, L. plantarum ATCC 14917 under initial acid and alkali stress was evaluated by analyzing auto-aggregation, protein adhesion and cell adhesion in vitro. Based on scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis, the morphology of the bacteria became thickset and the thickness of their cell walls decreased under initial alkali stress. The fold changes of auto-aggregation, adhere to mucin and HT-29 cell lines of L. plantarum ATCC 14917 in the acid group were increased by 1.141, 1.125 and 1.156, respectively. But decreased significantly in the alkali group (fold changes with 0.842, 0.728 and 0.667). Adhesion-related protein increased in the acid group but declined in the alkali group at the mRNA expression level according to real time polymerase chain reaction (RT-PCR) analysis. The changes in the metabolite profiles of L. plantarum ATCC 14917 were characterized using Ultra-Performance Liquid Chromatography-Electrospray ionization-Quadrupole-Time of Flight-mass spectrometry (UPLS-ESI-Q-TOF-MS). In the alkali group, the content of a lot of substances involved in the energy and amino acid metabolism decreased, but the content of some substances involved in the energy metabolism was slightly increased in the acid group. These findings demonstrate that energy metabolism is positively correlated with the adhesion ability of L. plantarum ATCC 14917. The amino-acids metabolism, especially the amino acids related to pH-homeostasis mechanisms (lysine, aspartic acid, arginine, proline and glutamic acid), showed an obvious effect on the adhesion ability of L. plantarum ATCC 14917. This investigation provides a better understanding of L. plantarum's adhesion mechanisms under initial pH stress.
植物乳杆菌的黏附能力影响其在人体胃肠道中的滞留时间，并影响与宿主的相互作用。在这项研究中，通过分析体外的自聚集、蛋白黏附和细胞黏附，评估了植物乳杆菌 ATCC 14917 在初始酸碱胁迫下的黏附活性与代谢变化之间的关系。基于扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析，发现初始碱胁迫下细菌变得厚实，细胞壁厚度减小。在酸组中，植物乳杆菌 ATCC 14917 的自聚集、黏附黏蛋白和 HT-29 细胞系的倍数变化分别增加了 1.141、1.125 和 1.156，但在碱组中显著下降（倍数变化分别为 0.842、0.728 和 0.667）。实时聚合酶链反应（RT-PCR）分析表明，黏附相关蛋白在酸组中增加，但在碱组中下降。根据超高效液相色谱-电喷雾电离-四极杆飞行时间质谱联用（UPLC-ESI-Q-TOF-MS）分析，植物乳杆菌 ATCC 14917 的代谢物谱发生了变化。在碱组中，许多参与能量和氨基酸代谢的物质含量下降，但在酸组中，一些参与能量代谢的物质含量略有增加。这些发现表明，能量代谢与植物乳杆菌 ATCC 14917 的黏附能力呈正相关。氨基酸代谢，特别是与 pH 稳态机制相关的氨基酸（赖氨酸、天冬氨酸、精氨酸、脯氨酸和谷氨酸），对植物乳杆菌 ATCC 14917 的黏附能力有明显影响。本研究为植物乳杆菌在初始 pH 胁迫下的黏附机制提供了更好的理解。
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