• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

山羊中经典型和 L 型非典型 BSE 的临床、病理和分子特征。

Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats.

机构信息

Centre of Animal Encephalopathies (CEA), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy.

Dipartimento di Scienze Veterinarie, Sezione Clinica Medica, University of Turin, Grugliasco (Turin), Italy.

出版信息

PLoS One. 2018 May 24;13(5):e0198037. doi: 10.1371/journal.pone.0198037. eCollection 2018.

DOI:10.1371/journal.pone.0198037
PMID:29795663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5968405/
Abstract

Monitoring of small ruminants for transmissible spongiform encephalopathies (TSEs) has recently become more relevant after two natural scrapie suspected cases of goats were found to be positive for classical BSE (C-BSE). C-BSE probably established itself in this species unrecognized, undermining disease control measures. This opens the possibility that TSEs in goats may remain an animal source for human prion diseases. Currently, there are no data regarding the natural presence of the atypical BSE in caprines. Here we report that C-BSE and L-type atypical BSE (L-BSE) isolates from bovine species are intracerebrally transmissible to goats, with a 100% attack rate and a significantly shorter incubation period and survival time after C-BSE than after L-BSE experimental infection, suggesting a lower species barrier for classical agentin goat. All animals showed nearly the same clinical features of disease characterized by skin lesions, including broken hair and alopecia, and abnormal mental status. Histology and immunohistochemistry showed several differences between C-BSE and L-BSE infection, allowing discrimination between the two different strains. The lymphoreticular involvement we observed in the C-BSE positive goats argues in favour of a peripheral distribution of PrPSc similar to classical scrapie. Western blot and other currently approved screening tests detected both strains in the goats and were able to classify negative control animals. These data demonstrate that active surveillance of small ruminants, as applied to fallen stock and/or healthy slaughter populations in European countries, is able to correctly identify and classify classical and L-BSE and ultimately protect public health.

摘要

最近，在发现两例疑似天然羊瘙痒病的山羊病例为经典牛海绵状脑病（C-BSE）阳性后，对小反刍动物进行传染性海绵状脑病（TSEs）监测变得更加重要。C-BSE 可能在未被察觉的情况下在该物种中建立起来，破坏了疾病控制措施。这使得 TSEs 在山羊中可能仍然是人类朊病毒病的动物来源。目前，关于山羊中天然存在非典型 BSE 的数据尚不清楚。在这里，我们报告说，来自牛种的 C-BSE 和 L 型非典型 BSE（L-BSE）分离株可通过脑内途径传播给山羊，具有 100%的攻击率，且在 C-BSE 感染后潜伏期和存活时间明显短于 L-BSE 实验感染，这表明经典病原体在山羊中的种间屏障较低。所有动物都表现出几乎相同的疾病临床特征，包括皮肤损伤，包括断毛和脱发，以及异常的精神状态。组织学和免疫组织化学显示 C-BSE 和 L-BSE 感染之间存在一些差异，允许区分两种不同的菌株。我们在 C-BSE 阳性山羊中观察到的淋巴网状组织受累表明，PrPSc 的外周分布类似于经典瘙痒病。Western blot 和其他目前批准的筛选试验在山羊中检测到了两种菌株，并能够对阴性对照动物进行分类。这些数据表明，对小反刍动物进行主动监测，如在欧洲国家应用于死亡牲畜和/或健康屠宰群体，能够正确识别和分类经典和 L-BSE，并最终保护公众健康。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/f9a976511af8/pone.0198037.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/b8620717d719/pone.0198037.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/313bf03f093c/pone.0198037.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/761945c49a4a/pone.0198037.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/463358031714/pone.0198037.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/ab51f94feaa1/pone.0198037.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/8326ebf63b2b/pone.0198037.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/798eeaf4f9a6/pone.0198037.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/7df6ecefb4e7/pone.0198037.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/4f6d1263184d/pone.0198037.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/f9a976511af8/pone.0198037.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/b8620717d719/pone.0198037.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/313bf03f093c/pone.0198037.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/761945c49a4a/pone.0198037.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/463358031714/pone.0198037.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/ab51f94feaa1/pone.0198037.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/8326ebf63b2b/pone.0198037.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/798eeaf4f9a6/pone.0198037.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/7df6ecefb4e7/pone.0198037.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/4f6d1263184d/pone.0198037.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46ff/5968405/f9a976511af8/pone.0198037.g010.jpg

相似文献

1
Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats.山羊中经典型和 L 型非典型 BSE 的临床、病理和分子特征。
PLoS One. 2018 May 24;13(5):e0198037. doi: 10.1371/journal.pone.0198037. eCollection 2018.
2
Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain.用来自英国的两个时间上分开的绵羊瘙痒病源接种牛后，对传染性海绵状脑病表型进行进一步表征。
BMC Res Notes. 2015 Jul 24;8:312. doi: 10.1186/s13104-015-1260-3.
3
Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie.经典 BSE 朊病毒从接受非典型/Nor98 瘙痒病挑战的无症状猪中出现。
Sci Rep. 2021 Aug 31;11(1):17428. doi: 10.1038/s41598-021-96818-2.
4
Experimentally induced bovine spongiform encephalopathy did not transmit via goat embryos.
J Gen Virol. 1999 Feb;80 ( Pt 2):517-524. doi: 10.1099/0022-1317-80-2-517.
5
Surveillance and simulation of bovine spongiform encephalopathy and scrapie in small ruminants in Switzerland.瑞士小反刍动物的牛海绵状脑病和羊痒病的监测和模拟。
BMC Vet Res. 2010 Apr 18;6:20. doi: 10.1186/1746-6148-6-20.
6
Clinical signs, histopathology and genetics of experimental transmission of BSE and natural scrapie to sheep and goats.牛海绵状脑病和自然痒病向绵羊和山羊实验性传播的临床症状、组织病理学及遗传学研究
Vet Rec. 2001 Feb 10;148(6):165-71. doi: 10.1136/vr.148.6.165.
7
A C-terminal protease-resistant prion fragment distinguishes ovine "CH1641-like" scrapie from bovine classical and L-Type BSE in ovine transgenic mice.一种C末端抗蛋白酶朊病毒片段在绵羊转基因小鼠中可区分绵羊“CH1641样”瘙痒病与牛经典型和L型牛海绵状脑病。
PLoS Pathog. 2008 Aug 29;4(8):e1000137. doi: 10.1371/journal.ppat.1000137.
8
Sheep prions with molecular properties intermediate between classical scrapie, BSE and CH1641-scrapie.具有介于经典羊瘙痒病、疯牛病和CH1641型羊瘙痒病之间分子特性的羊朊病毒。
Prion. 2014;8(4):296-305. doi: 10.4161/19336896.2014.983396.
9
Molecular typing of protease-resistant prion protein in transmissible spongiform encephalopathies of small ruminants, France, 2002-2009.2002-2009 年法国小反刍动物传染性海绵状脑病的朊病毒蛋白抗蛋白酶型分子分型。
Emerg Infect Dis. 2011 Jan;17(1):55-63. doi: 10.3201/eid1701.100891.
10
Histopathological studies of "CH1641-like" scrapie sources versus classical scrapie and BSE transmitted to ovine transgenic mice (TgOvPrP4).“CH1641 样”羊瘙痒病源的组织病理学研究与经典羊瘙痒病和 BSE 经绵羊转基因小鼠（TgOvPrP4）传播的比较。
PLoS One. 2011;6(7):e22105. doi: 10.1371/journal.pone.0022105. Epub 2011 Jul 13.

引用本文的文献

1
Infectious prions in brains and muscles of domestic pigs experimentally challenged with the BSE, scrapie, and CWD agents.用牛海绵状脑病、羊瘙痒病和慢性消耗病病原体对家猪进行实验性攻击后，其大脑和肌肉中的传染性朊病毒
mBio. 2025 Sep 10;16(9):e0180025. doi: 10.1128/mbio.01800-25. Epub 2025 Aug 18.
2
RT-QuIC detection of pathological prion protein in subclinical goats following experimental oral transmission of L-type BSE.经口感染 L 型 BSE 后处于亚临床状态的山羊的 RT-QuIC 检测到病理性朊病毒蛋白。
BMC Res Notes. 2021 Dec 7;14(1):442. doi: 10.1186/s13104-021-05859-3.
3
Early and Pre-Clinical Detection of Prion Seeding Activity in Cerebrospinal Fluid of Goats using Real-Time Quaking-Induced Conversion Assay.

本文引用的文献

1
EU-approved rapid tests might underestimate bovine spongiform encephalopathy infection in goats.
J Vet Diagn Invest. 2017 Mar;29(2):232-236. doi: 10.1177/1040638716688045. Epub 2017 Jan 9.
2
L-BSE experimentally transmitted to sheep presents as a unique disease phenotype.实验性传播给绵羊的L型疯牛病呈现出独特的疾病表型。
Vet Res. 2016 Nov 8;47(1):112. doi: 10.1186/s13567-016-0394-1.
3
L-type bovine spongiform encephalopathy in genetically susceptible and resistant sheep: changes in prion strain or phenotypic plasticity of the disease-associated prion protein?L 型牛海绵状脑病在遗传易感和抗性绵羊中的表现：朊病毒株的变化还是与疾病相关的朊病毒蛋白的表型可塑性？
使用实时液滴震荡转换分析方法，在山羊脑脊液中早期和临床前检测朊病毒接种活性。
Sci Rep. 2019 Apr 16;9(1):6173. doi: 10.1038/s41598-019-42449-7.
J Infect Dis. 2014 Mar;209(6):950-9. doi: 10.1093/infdis/jit596. Epub 2013 Nov 11.
4
Distribution of abnormal prion protein in a sheep affected with L-type bovine spongiform encephalopathy.感染L型牛海绵状脑病的绵羊中异常朊病毒蛋白的分布
J Comp Pathol. 2013 Jul;149(1):113-8. doi: 10.1016/j.jcpa.2012.11.231. Epub 2012 Dec 27.
5
EU-approved rapid tests for bovine spongiform encephalopathy detect atypical forms: a study for their sensitivities.欧盟批准的用于检测牛海绵状脑病的快速检测方法可检测到非典型形式：一项关于其敏感性的研究。
PLoS One. 2012;7(9):e43133. doi: 10.1371/journal.pone.0043133. Epub 2012 Sep 11.
6
Susceptibility of young sheep to oral infection with bovine spongiform encephalopathy decreases significantly after weaning.幼羊在断奶后对牛海绵状脑病的经口感染易感性显著降低。
J Virol. 2012 Nov;86(21):11856-62. doi: 10.1128/JVI.01573-12. Epub 2012 Aug 22.
7
Resistance to classical scrapie in experimentally challenged goats carrying mutation K222 of the prion protein gene.携带朊病毒蛋白基因 K222 突变的实验性攻毒山羊对经典瘙痒病的抗性。
Vet Res. 2012 Feb 1;43(1):8. doi: 10.1186/1297-9716-43-8.
8
Isolation of prion with BSE properties from farmed goat.从养殖山羊中分离出具有 BSE 特性的朊病毒。
Emerg Infect Dis. 2011 Dec;17(12):2253-61. doi: 10.3201/eid1712.110333.
9
Monitoring of clinical signs in goats with transmissible spongiform encephalopathies.监测患有传染性海绵状脑病的山羊的临床症状。
BMC Vet Res. 2010 Mar 4;6:13. doi: 10.1186/1746-6148-6-13.
10
Three serial passages of bovine spongiform encephalopathy in sheep do not significantly affect discriminatory test results.牛海绵状脑病在绵羊体内连续传代三次对鉴别试验结果无显著影响。
J Gen Virol. 2009 Mar;90(Pt 3):764-768. doi: 10.1099/vir.0.005983-0.