Skopik S D, Cain W, Patel N G, Newswanger D L
School of Life and Health Sciences, University of Delaware, Newark 19716.
J Biol Rhythms. 1986 Summer;1(2):145-50. doi: 10.1177/074873048600100205.
A 12-hr dark period, at a temperature high enough to permit time measurement to occur, is necessary for maximal induction of larval diapause in the European corn borer, Ostrinia nubilalis. In the present study, induction of diapause only occurred in a periodic environment. This was in the form of certain (1) light-dark (LD) cycles at a constant temperature; (2) thermoperiods in constant darkness (DD), but not constant illumination (LL); and (3) LD cycles with concurrent thermoperiods. A light-break experiment protocol, in which the pulses systematically scan the cold and warm phases of a thermoperiod in DD, is discussed as a way of helping clarify how seasonal time measurement is effected in Ostrinia.
对于欧洲玉米螟（Ostrinia nubilalis）幼虫滞育的最大诱导而言，需要一个12小时的黑暗期，且温度要足够高以允许进行时间测量。在本研究中，滞育诱导仅发生在周期性环境中。其形式为：（1）在恒定温度下的特定光暗（LD）循环；（2）在恒定黑暗（DD）而非恒定光照（LL）条件下的温度周期；以及（3）伴有同期温度周期的LD循环。文中讨论了一种光中断实验方案，即脉冲系统地扫描DD中温度周期的冷期和暖期，以此作为一种有助于阐明欧洲玉米螟如何进行季节性时间测量的方法。
