Page T L
Department of General Biology, Vanderbilt University, Nashville, Tennessee 37235.
J Biol Rhythms. 1987 Spring;2(1):23-34. doi: 10.1177/074873048700200103.
Serotonin, a putative neurotransmitter in insects, was found to cause consistent phase shifts of the circadian rhythm of locomotor activity of the cockroach Leucophaea maderae when administered during the early subjective night as a series of 4-microliters pulses (one every 15 min) for either 3 or 6 hr. Six-hour treatments with dopamine also caused significant phase shifts during the early subjective night, but 3-hr treatments with dopamine had no phase-shifting effect. Other substances tested in early subjective night (norepinephrine, octopamine, gamma-aminobutyric acid, glutamate, carbachol, histamine, tryptophan, tryptamine, N-acetyl serotonin, or 5-hydroxyindole-3-acetic acid) did not consistently cause phase shifts. The phase-shifting effect of serotonin was found to be phase-dependent. The phase response curve (PRC) for serotonin treatments was different from the PRC for light. Like light, serotonin caused phase delays in the late subjective day and early subjective night, but serotonin did not phase-shift rhythms when tested at phases where light causes phase advances.
血清素是昆虫体内一种假定的神经递质，研究发现，当在主观夜晚早期以一系列4微升脉冲（每15分钟一个）的形式给药3或6小时时，它会使马德拉蜚蠊的运动活动昼夜节律产生持续的相位偏移。在主观夜晚早期用多巴胺进行6小时的处理也会导致显著的相位偏移，但用多巴胺进行3小时的处理则没有相位偏移效果。在主观夜晚早期测试的其他物质（去甲肾上腺素、章鱼胺、γ-氨基丁酸、谷氨酸、卡巴胆碱、组胺、色氨酸、色胺、N-乙酰血清素或5-羟基吲哚-3-乙酸）并没有持续引起相位偏移。血清素的相位偏移效应被发现是依赖相位的。血清素处理的相位响应曲线（PRC）与光的PRC不同。与光一样，血清素在主观白天后期和主观夜晚早期会导致相位延迟，但在光会引起相位提前的相位进行测试时，血清素不会使节律产生相位偏移。
