Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Poult Sci. 2018 Aug 1;97(8):2647-2653. doi: 10.3382/ps/pey113.
In the present study, the pathogenicity of fowl adenovirus (FAdV) serotype 4 strain HB1501 was systematically determined by using different doses and routes of inoculation in chickens and assessed the immune efficacy induced by fowl adenovirus serotype 4 (FAdV-4) strain SB15 in a vaccination trial. The results revealed that strain HB1501 was highly virulent in chickens, inflicting 30 to 100% mortality when administered by intramuscular and intravenous routes, and its pathogenicity was influenced markedly by the inoculation route. The inactivated oil-emulsion FAdV-4 vaccine derived from strain SB15 completely protected specific pathogen-free chickens against morbidity and mortality following infection with the virulent FAdV-4 strain HB1501. Importantly, no obvious clinical signs or gross lesions were observed and high antibody levels against FAdV were measured in the SB15-vaccinated chickens, indicating the potential of this strain to become an alternative vaccine candidate. This study provides detailed information on the pathogenicity of FAdV-4, which should be of great value to the control of hepatitis-hydropericardium syndrome.
在本研究中，通过在鸡中使用不同剂量和接种途径，系统地确定了禽腺病毒（FAdV）血清型 4 株 HB1501 的致病性，并评估了禽腺病毒血清型 4（FAdV-4）株 SB15 在疫苗接种试验中诱导的免疫效果。结果表明，HB1501 株在鸡中具有高度致病性，通过肌肉内和静脉途径接种时，死亡率为 30%至 100%，其致病性明显受接种途径的影响。源自 SB15 株的禽腺病毒-4 型灭活油乳剂疫苗完全保护无特定病原体鸡免受强毒 FAdV-4 株 HB1501 感染引起的发病率和死亡率。重要的是，在接种 SB15 的鸡中未观察到明显的临床症状或大体病变，并且针对 FAdV 的抗体水平很高，表明该株有成为替代疫苗候选株的潜力。本研究提供了关于 FAdV-4 致病性的详细信息，这对于控制肝炎-心包积水综合征具有重要价值。
