Seckinger Anja, Bähr-Ivacevic Tomi, Benes Vladimir, Hose Dirk
Labor für Myelomforschung, Medizinische Klinik V, Universitätsklinikum Heidelberg, Heidelberg, Germany.
Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
Methods Mol Biol. 2018;1792:97-115. doi: 10.1007/978-1-4939-7865-6_7.
RNA sequencing is a recently developed approach for transcriptome profiling with several advantages over gene expression profiling using DNA microarrays. Here we describe a RNA-sequencing protocol optimized for low-input analysis of total RNA from CD138 purified plasma cells from myeloma patients which is applicable in clinical routine.
RNA测序是一种最近开发的用于转录组分析的方法，与使用DNA微阵列进行基因表达分析相比具有多个优势。在此，我们描述了一种针对骨髓瘤患者CD138纯化浆细胞的总RNA低输入量分析而优化的RNA测序方案，该方案适用于临床常规操作。
