Department of Physiology and Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Cell Physiol. 2018 Nov;233(11):8908-8918. doi: 10.1002/jcp.26814. Epub 2018 May 24.
Umbelliprenin (UMB) has shown various pharmacological properties in vitro. We investigated the antineoplastic and immunostimulatory effects of UMB in 4T1 mammary-tumor-bearing mice. Two-hundred microliter of UMB (12.5 mg/ml) was intraperitoneally administrated to healthy and tumor-bearing female Balb/c mice for a period of 18 days. Data was analyzed using GraphPad Prism 5 software for Windows (version 5, La Jolla, CA). UMB caused a significant decrease in tumor size (P < 0.01). Serum interferon gamma (IFNγ) was augmented in both healthy and tumor-bearing animals (P < 0.01), and IL-4 declined in healthy animals (P < 0.01) treated with UMB. Expressions of Ki-67, VEGF, CD31, MMP2, MMP9, VCAM1, and NF-κB were significantly decreased in tumors from UMB-treated animals (P < 0.001), whereas E-Cadherin and TNFR1 expressions were markedly increased (P < 0.001). The rates of liver and lung metastases in UMB-administrated animals were smaller compared to the control. UMB can potently inhibit tumor growth, angiogenesis, metastasis, and inflammation and potentiate an antitumor immune response in vivo. However, further investigations are required to evaluate the UMB mechanisms of action in cancerous cells.
Umbelliprenin (UMB) 在体外显示出多种药理特性。我们研究了 UMB 在 4T1 乳腺癌荷瘤小鼠中的抗肿瘤和免疫刺激作用。将 200 μl UMB（12.5 mg/ml）腹腔内给予健康和荷瘤雌性 Balb/c 小鼠，持续 18 天。使用 GraphPad Prism 5 软件 for Windows（版本 5，La Jolla，CA）分析数据。UMB 导致肿瘤体积显著减小（P < 0.01）。UMB 处理的健康和荷瘤动物的血清干扰素 γ（IFNγ）均增加（P < 0.01），而健康动物的 IL-4 下降（P < 0.01）。来自 UMB 处理动物的肿瘤中 Ki-67、VEGF、CD31、MMP2、MMP9、VCAM1 和 NF-κB 的表达显著降低（P < 0.001），而 E-Cadherin 和 TNFR1 的表达明显增加（P < 0.001）。与对照组相比，UMB 给药动物的肝和肺转移率较小。UMB 能够强烈抑制肿瘤生长、血管生成、转移和炎症，并增强体内抗肿瘤免疫反应。然而，需要进一步研究来评估 UMB 在癌细胞中的作用机制。
