Kalíková Květa, Voborná Markéta, Tesařová Eva
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.
Electrophoresis. 2018 Aug;39(16):2144-2151. doi: 10.1002/elps.201800141. Epub 2018 Jul 8.
The chromatographic behavior of new biogenic purine nucleosides in hydrophilic interaction liquid chromatography was examined on three different stationary phases, namely bare silica, and amide- and cyclofructan-based stationary phases. The effects of buffer concentration, pH and acetonitrile-to-aqueous-part ratio in the mobile phase on retention and peak shape were assessed. The retention coefficients and peak symmetry values substantially differed with respect to analytes´ structures, stationary phase properties and mobile phase composition. The bare silica column was unsuitable for these compounds under the chromatographic conditions tested due to very broad and asymmetrical peaks. Furthermore, the cyclofructan-based stationary phase provided almost Gaussian peak shapes of all deazapurine nucleosides under most conditions tested. Therefore, the cyclofructan-based stationary phase is the most suitable choice for the chromatographic analysis of nucleosides.
在三种不同的固定相上，即裸硅胶、酰胺基和环糊精基固定相，考察了新型生物嘌呤核苷在亲水作用液相色谱中的色谱行为。评估了流动相中缓冲液浓度、pH值以及乙腈与水相比例对保留时间和峰形的影响。保留系数和峰对称值因分析物结构、固定相性质和流动相组成的不同而有显著差异。在测试的色谱条件下，裸硅胶柱由于峰非常宽且不对称而不适用于这些化合物。此外，在大多数测试条件下，环糊精基固定相能使所有脱氮嘌呤核苷呈现几乎高斯峰形。因此，环糊精基固定相是核苷色谱分析的最合适选择。
