Xue Dong, Huang Xiang Dong, Yang Rui Xian, Wang Zu Hua
Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, Henan, China.
Ying Yong Sheng Tai Xue Bao. 2018 May;29(5):1645-1652. doi: 10.13287/j.1001-9332.201805.035.
A strain PSPSA1 with strong phosphate-solubilizing ability was isolated from the rhi-zosphere of tree peony. This strain was identified based on morphological characteristics, physiological and biochemical experiments, and 16S rDNA sequence analysis. Furthermore, its genetic stabi-lity and phosphate-solubilizing characteristics were investigated. Strain PSPSA1 was identified as the actinomycete Streptomyces albireticuli and showed high genetic stability. The order of phosphate solubilization capacity (PSC) in different phosphorus-source culture media was calcium phosphate (158.5 mg·L) > aluminum phosphate (139.9 mg·L) > ferric phosphate (127.7 mg·L) > lecithin (45.6 mg·L). The PSC and pH were significantly negatively correlated in inorganic phosphorus solution, but were not correlated in organic phosphorus solution. When using different carbon sources, PSC was in the order of lactose > glucose > maltose > fructose > sucrose > starch > cellulose. For different nitrogen sources, PSC was in the order of peptone > ammonium nitrate > ammonium sulfate > potassium nitrate > urea. Using glucose as a carbon source and peptone as a nitrogen source, strain PSPSA1 achieved the highest PSC of 202.6 mg·L. After incubated for 60 days, the available phosphorus content in soil with a single application of strain PSPSA1 increased by 68.2% compared with control, and the available phosphorus content in soil with mixed application of strain PSPSA1 and organic fertilizer increased by 76.7% compared with a single application of organic fertilizer. The results indicated that the strain PSPSA1 had a strong ability to dissolve insoluble phosphorus and its mixed application with organic fertilizer improved the phosphorus solubilization. Thus, PSPSA1 is a promising microbial resource for the production of high-efficiency biological phosphorus fertilizer.
从牡丹根际分离出一株具有较强解磷能力的菌株PSPSA1。基于形态特征、生理生化实验和16S rDNA序列分析对该菌株进行了鉴定。此外，还研究了其遗传稳定性和解磷特性。菌株PSPSA1被鉴定为放线菌白网链霉菌，具有较高的遗传稳定性。不同磷源培养基中的解磷能力（PSC）顺序为：磷酸钙（158.5 mg·L）＞磷酸铝（139.9 mg·L）＞磷酸铁（127.7 mg·L）＞卵磷脂（45.6 mg·L）。在无机磷溶液中，PSC与pH呈显著负相关，但在有机磷溶液中不相关。使用不同碳源时，PSC顺序为：乳糖＞葡萄糖＞麦芽糖＞果糖＞蔗糖＞淀粉＞纤维素。对于不同氮源，PSC顺序为：蛋白胨＞硝酸铵＞硫酸铵＞硝酸钾＞尿素。以葡萄糖为碳源、蛋白胨为氮源时，菌株PSPSA1的PSC最高，为202.6 mg·L。单独施用菌株PSPSA1的土壤中有效磷含量在培养60天后比对照增加了68.2%，菌株PSPSA1与有机肥混合施用的土壤中有效磷含量比单独施用有机肥增加了76.7%。结果表明，菌株PSPSA1具有较强的溶解难溶性磷的能力，其与有机肥混合施用提高了解磷效果。因此，PSPSA1是一种有潜力的生产高效生物磷肥的微生物资源。
