Fan Yan Ping, Cheng Xiao Fang, Wang Hong Min, Zhang Yao Wen, Zhang Xian Hong
College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
Ying Yong Sheng Tai Xue Bao. 2018 May;29(5):1660-1666. doi: 10.13287/j.1001-9332.201805.040.
To clarify the effects of protease inhibitors on the activities of midgut proteases in Callosobruchus chinensis (L.) larvae, the inhibitory effects of four kinds of protease inhibitors on the activities of midgut proteases of C. chinensis larvae were examined in vitro and in feeding conditions with indoor artificial insect inoculation. The activities of total protease, trypsin-like enzyme and chymotrypsin-like enzyme in the midgut of C. chinensis larvae fed on artificial mung bean that contained different contents of mung bean types in inhibitor (MBTI) were examined. The results showed that those four protease inhibitors had significant inhibitory effects on the activities of total protease, trypsin-like enzyme and chymotrypsin-like enzyme in C. chinensis larvae. The inhibitory effect was more significant when the concentration was higher. Among those inhibitors, the inhibitory effect of 20 μg·mL MBTI on three kinds of enzyme activities was the strongest, with reduction of 62.5%, 41.2% and 38.7%, respectively. Ovomucoid inhibitor (OI) had the lowest inhibitory effect. The activities of three enzymes in the midgut were also inhibited by C. chinensis larvae fed with artificial mung bean containing different inhibitors. The activities of three enzymes increased with the prolongation of the instar after feeding, but they were significantly lower than that in control. The inhibitory effect of MBTI was the strongest. The inhibitory effect on the activities of total protease and trypsin-like enzyme was gradually enhanced with the increases of MBTI contents when C. chinensis larvae were fed with artificial mung bean with different contents MBTI. The inhibitory effect on chymotrypsin-like enzyme activity was not significant. When the content of MBTI was up to 20%, the activity of chymotrypsin-like enzyme was obviously inhibited.
为阐明蛋白酶抑制剂对绿豆象幼虫中肠蛋白酶活性的影响，在体外以及通过室内人工接虫的饲养条件下，检测了4种蛋白酶抑制剂对绿豆象幼虫中肠蛋白酶活性的抑制作用。检测了取食含不同抑制剂含量的绿豆象人工饲料（MBTI）的绿豆象幼虫中肠总蛋白酶、类胰蛋白酶和类胰凝乳蛋白酶的活性。结果表明，这4种蛋白酶抑制剂对绿豆象幼虫中肠总蛋白酶、类胰蛋白酶和类胰凝乳蛋白酶的活性均有显著抑制作用，浓度越高抑制作用越显著。其中，20 μg·mL MBTI对3种酶活性的抑制作用最强，分别降低了62.5%、41.2%和38.7%。卵类黏蛋白抑制剂（OI）的抑制作用最低。取食含不同抑制剂的绿豆象人工饲料的幼虫，其3种酶的活性也受到抑制。取食后3种酶的活性均随龄期延长而升高，但均显著低于对照。MBTI的抑制作用最强。当绿豆象幼虫取食含不同MBTI含量的人工饲料时，MBTI对总蛋白酶和类胰蛋白酶活性的抑制作用随MBTI含量的增加而逐渐增强，对类胰凝乳蛋白酶活性的抑制作用不显著，当MBTI含量达20%时，类胰凝乳蛋白酶活性受到明显抑制。
