Ba L, Du J T, Cai S Y, Li J, Zhou P, Liu Y F
Department of Otorhinolaryngology,People's Hospital of Tibet Autonomous Region,Lhasa,850099,China.
The Upper Airways Research Laboratory of Department of Otorhinolaryngology Head and Neck Surgery of West China Hospital.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Aug 20;30(16):1302-1307. doi: 10.13201/j.issn.1001-1781.2016.16.010.
The aim of this study is to study the different immune-type of polyps and valuate mucosal inflammatory pattern and remodeling features between in IL-5 positive vs.IL-17 positive nasal polyps.Nasal polyp or nasal turbinate tissue was obtained from 88 CRSwNP patients during endonasal sinus surgery or 18 non-atopic control subjects during septoplasty,respectively.Assessment of pro-inflammatory cytokines and mediators by ELISA.Additionally,the distribution of IL-5 positive or IL-17 positive cells and inflammatory cells(eosinophil,neutrophil,etc.) were examined using immunohistochemistry(IHC).Overall more than half amount of polyp tissue did not express any TH cells key cytokine.However there are 21% polyp present IL-5 positive and 16% of IL-17 positive as well as 9% of IFN-γ positives.Amount of them that IL-17 positive polyps by synthesis of mediators promoting neutrophilic inflammation[myeloperoxidase(MPO),IL-1β,IL-6 and IL-8)] and staining infiltration of MPO positive with IL-17 positive cells,whereas IL-5 positive nasal polyps were characterized by synthesis of mediators promoting eosinophilic inflammation(IL-5,ECP,TIgE,and SAE-IgE) and infiltrating of eosinophils and IL-5 positive cells.Meanwhile TGF-β1 and MMP7 protein levels enhanced in IL-17 positive polyps and decreased in IL-5 positive than control.Nasal polyps presenting as different immune types and there were characterized with different inflammatory and remodeling patterns.Amount of them that IL-17 positive polyps presenting as a neutrophilic inflammation with remodeling biased.Whereas IL-5 positive nasal polyps were characterized by eosinophilic inflammation and absence of remodeling tendency.
本研究旨在探讨不同免疫类型的鼻息肉，并评估白细胞介素-5（IL-5）阳性与白细胞介素-17（IL-17）阳性鼻息肉之间的黏膜炎症模式和重塑特征。分别从88例慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）患者的鼻内镜鼻窦手术中获取鼻息肉或鼻甲组织，以及18例非特应性对照受试者的鼻中隔成形术中获取组织。通过酶联免疫吸附测定（ELISA）评估促炎细胞因子和介质。此外，采用免疫组织化学（IHC）检测IL-5阳性或IL-17阳性细胞以及炎性细胞（嗜酸性粒细胞、中性粒细胞等）的分布。总体而言，超过半数的息肉组织未表达任何TH细胞关键细胞因子。然而，有21%的息肉呈IL-5阳性，16%呈IL-17阳性，还有9%呈干扰素-γ（IFN-γ）阳性。其中，IL-17阳性鼻息肉通过促进中性粒细胞炎症的介质合成[髓过氧化物酶（MPO）、IL-1β、IL-6和IL-8]以及MPO阳性与IL-17阳性细胞的染色浸润来表征，而IL-5阳性鼻息肉的特征是促进嗜酸性粒细胞炎症的介质合成（IL-5、嗜酸性粒细胞阳离子蛋白、总IgE和特异性IgE）以及嗜酸性粒细胞和IL-5阳性细胞的浸润。同时，与对照组相比，IL-17阳性息肉中转化生长因子-β1（TGF-β1）和基质金属蛋白酶7（MMP7）蛋白水平升高，而IL-5阳性息肉中则降低。鼻息肉呈现出不同的免疫类型，并具有不同的炎症和重塑模式。其中，IL-17阳性鼻息肉表现为以重塑为偏向性的中性粒细胞炎症。而IL-5阳性鼻息肉的特征是嗜酸性粒细胞炎症且无重塑倾向。
