Chen S, Zhou J Z, Weng Y H, Liu H G, Long X B
Department of Otolaryngology Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China.
Department of Otolaryngology Head and Neck Surery，the First People's Hospital of Shunde.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Dec;30(23):1881-1884. doi: 10.13201/j.issn.1001-1781.2016.23.011.
To investigate effect of hydrogen peroxide (H₂O₂) in the lateral line hair cell growth and regeneration after damage on zebrafish. Select 5 dpf zebrafish, each group of 10, randomly divided into A control group: the system of water culture. B H₂O₂ group: 10 μmol/L, 20 μmol/L H₂O₂ solution to replace three times a day. C neomycin group: treatment with system water after 1 h culture by 200 μmol/L neomycin. D neomycin + H₂O₂ group: 20 μmol/L H₂O₂ solution to replace three times a day after 200 μmol/L neomycin treatment for 1 h. E cisplatin group: treatment with system water after 3 h culture by 1 000 μmol/L cisplatin. F cisplatin + H₂O₂ group: 20 μmol/L H₂O₂ solution to replace three times a day after 1 000 μmol/L cisplatin treatment for 3 h. Each group in H₂O₂ treatment for 0 h, 24 h, 48 h was marked their hair cells by immunofluorescence method and count the P1, P7, P8 neuromasts under the fluorescence microscope. Repeat 3 times. The number of hair cells on P1, P7, P8 three neuramasts among 5 to 7 dpf zebrafish were 9.364±0.901(=11),9.645±0.598(=15),9.922±0.862(=13), no obvious difference (>0.05); 10μmol/L, 20μmol/L H₂O₂ treated zebrafish for 48 h, the numbers were 11.540±0.741,11.905±0.607,compaired with the control group(10.841±0.389), <0.05; neomycin+ H₂O₂ 48 h and neomycin 48 h respectively were 10.600±0.689,8.767±0.603, <0.01; cisplatin+ H₂O₂ 48 h and cisplatin 48 h were 5.967±1.086,5.633±1.548, >0.05. 20 μmol/L H₂O₂ promotes the development of lateral line hair cells of zebrafish; H₂O₂ promotes the regeneration of the lateral line hair cells after injury of neomycin, but not cisplatin.
为研究过氧化氢（H₂O₂）对斑马鱼侧线毛细胞损伤后生长和再生的影响。选取5日龄斑马鱼，每组10条，随机分为：A对照组：水培体系。B H₂O₂组：用10μmol/L、20μmol/L H₂O₂溶液每天更换3次。C新霉素组：用200μmol/L新霉素培养1小时后用体系水培养。D新霉素+H₂O₂组：用200μmol/L新霉素处理1小时后，用20μmol/L H₂O₂溶液每天更换3次。E顺铂组：用1000μmol/L顺铂培养3小时后用体系水培养。F顺铂+H₂O₂组：用1000μmol/L顺铂处理3小时后，用20μmol/L H₂O₂溶液每天更换3次。对H₂O₂处理0小时、24小时、48小时的每组斑马鱼用免疫荧光法标记其毛细胞，并在荧光显微镜下计数P1、P7、P8神经丘。重复3次。5至7日龄斑马鱼P1、P7、P8三个神经丘上的毛细胞数量分别为9.364±0.901（n = 11）、9.645±0.598（n = 15）、9.922±0.862（n = 13），无明显差异（>0.05）；用10μmol/L、20μmol/L H₂O₂处理斑马鱼48小时后，数量分别为11.540±0.741、11.905±0.607，与对照组（10.841±0.389）相比，<0.05；新霉素+H₂O₂组48小时和新霉素组48小时分别为10.600±0.689、8.767±0.603，<0.01；顺铂+H₂O₂组48小时和顺铂组48小时分别为5.967±1.086、5.633±1.548，>0.05。20μmol/L H₂O₂促进斑马鱼侧线毛细胞的发育；H₂O₂促进新霉素损伤后侧线毛细胞的再生，但不促进顺铂损伤后侧线毛细胞的再生。
