Nakano K, Fujimoto S, Tokita H
Shizuoka Sena Hospital, Japan.
In Vivo. 1988 May-Aug;2(3-4):247-52.
Antitumor activity of ascorbic acid in combination with antitumor agents (mitomycin C and 5-fluorouracil) was examined against subcutaneously implanted Lewis lung carcinoma-bearing C57BL/6 mice by feeding them an ascorbic acid-deficient diet. The mice were divided into four groups: group 1 received intraperitoneally mitomycin C (2 mg/kg) and 5-fluorouracil (50 mg/kg) once a week for four weeks beginning from the day after implantation of tumors, as well as ascorbic acid (1000 mg/kg) twice a week for the same four weeks; group 2 received only mitomycin C and 5-fluorouracil; group 3 received only ascorbic acid; group 4 received a vehicle (physiological saline). Tumor growth of group 1 compared with the other three groups, and that of group 2 compared with groups 3 and 4, was significantly inhibited by day 13 post implantation. Histological examinations of tumor tissues at 10 days after implantation of tumors already showed degenerative changes which indicated these antitumor effects.
通过给皮下植入携带Lewis肺癌的C57BL/6小鼠喂食缺乏抗坏血酸的饮食，研究了抗坏血酸与抗肿瘤药物（丝裂霉素C和5-氟尿嘧啶）联合使用的抗肿瘤活性。小鼠被分为四组：第1组从肿瘤植入后的第二天开始，每周一次腹腔注射丝裂霉素C（2 mg/kg）和5-氟尿嘧啶（50 mg/kg），共四周，同时在相同的四周内每周两次给予抗坏血酸（1000 mg/kg）；第2组仅接受丝裂霉素C和5-氟尿嘧啶；第3组仅接受抗坏血酸；第4组接受赋形剂（生理盐水）。植入后第13天，第1组的肿瘤生长与其他三组相比，以及第2组的肿瘤生长与第3组和第4组相比，均受到显著抑制。肿瘤植入后10天对肿瘤组织进行的组织学检查已经显示出退行性变化，这表明了这些抗肿瘤作用。
