Nageshwari B, Merugu Ramchander
Department of Biotechnology, Government College Autonomous, Rajamahendravaram, Andhra Pradesh, India.
Department of Biochemistry, Mahatma Gandhi University, Nalgonda, Telangana, India.
Indian J Cancer. 2017 Jul-Sep;54(3):566-571. doi: 10.4103/ijc.IJC_349_17.
Multiple myeloma (MM) is a B-cell malignancy accounting for 0.8% of all cancer deaths globally. This malignancy is characterized by lytic bone disease renal insufficiency, anemia, hypercalcemia, and immunodeficiency. The myeloma cells have enhanced expression of CD138. CD138 is a transmembrane heparin sulfate glycoprotein expressed on different types of adherent and nonadherent cells.CD138 is used as a standard marker for identification of tumor cells.
Despite introduction of many therapeutic agents, the management of multiple myeloma (MM) remains a challenge and search for new therapeutic agents is in progress. In this study, we attempted to evaluate the effect of an alkaline phosphatase inhibitor, levamisole on expression of CD138, and level of interleukin-6 (IL-6) in human MM cell lines RPMI 8226 and U266 B1.
U266B1 and RPMI 8226 cell lines were obtained from the National Centre for Cell Sciences, Pune. Alkaline phosphatase assay, Interleukin-6 assay and CD138 expression on myeloma cells by flow cytometry were investigated when the cells were exposed to Levamisole.
Levamisole-mediated growth inhibition of myeloma cells in vitro is associated with a loss of CD138 and increased IL-6 secretion. The increased secretion of IL-6 by myeloma cells could be an attempt to protect themselves from apoptosis.
Levamisole inhibited CD138 expression and affected the levels of IL-6 in a dose-dependent manner. The results of the present study add new dimension to levamisole's mode of action as inhibitor of CD138 and IL-6 and as an antiapoptotic agent.
多发性骨髓瘤（MM）是一种B细胞恶性肿瘤，占全球所有癌症死亡人数的0.8%。这种恶性肿瘤的特征是溶骨性骨病、肾功能不全、贫血、高钙血症和免疫缺陷。骨髓瘤细胞中CD138的表达增强。CD138是一种跨膜硫酸乙酰肝素糖蛋白，在不同类型的贴壁细胞和非贴壁细胞上表达。CD138用作鉴定肿瘤细胞的标准标志物。
尽管引入了许多治疗药物，但多发性骨髓瘤（MM）的治疗仍然是一项挑战，目前正在寻找新的治疗药物。在本研究中，我们试图评估碱性磷酸酶抑制剂左旋咪唑对人MM细胞系RPMI 8226和U266 B1中CD138表达及白细胞介素-6（IL-6）水平的影响。
U266B1和RPMI 8226细胞系购自印度浦那国家细胞科学中心。当细胞暴露于左旋咪唑时，研究碱性磷酸酶测定、白细胞介素-6测定以及通过流式细胞术检测骨髓瘤细胞上的CD138表达。
左旋咪唑在体外介导的骨髓瘤细胞生长抑制与CD138的丧失和IL-6分泌增加有关。骨髓瘤细胞IL-6分泌增加可能是其试图保护自身免于凋亡的一种尝试。
左旋咪唑以剂量依赖方式抑制CD138表达并影响IL-6水平。本研究结果为左旋咪唑作为CD138和IL-6抑制剂以及抗凋亡剂的作用方式增添了新的维度。
