Subhani Zuhaib, Daniel Barbara, Frascione Nunzianda
Department of Analytical, Environmental and Forensic Sciences, School of Population Health & Environmental Sciences, Faculty of Life Sciences & Medicine, King's College London, 150 Stamford Street, London, SE1 9NH, U.K.
J Forensic Sci. 2019 Jan;64(1):201-206. doi: 10.1111/1556-4029.13830. Epub 2018 May 25.
This study evaluated the compatibility of the most common enhancement methods and lifting techniques with DNA profiling. Emphasis is placed on modern lifting techniques (i.e., gelatin lifters and Isomark™) and historical fingerprint lifts for which limited research has been previously conducted. A total of 180 fingerprints were deposited on a glass surface, enhanced, lifted, and processed for DNA typing. DNA could be extracted and profiled for all the powders and lifts tested and from both groomed fingerprints and natural prints with no significant difference in the percentage of profile recovered. DNA profiles could also be obtained from historical fingerprint lifts (79.2% of 72 lifts) with one or more alleles detected. These results demonstrate the compatibility between different powder/lift combinations and DNA profiling therefore augmenting the evidential value of fingerprints in forensic casework.
本研究评估了最常见的增强方法和提取技术与DNA分型的兼容性。重点关注现代提取技术(即明胶提取器和Isomark™)以及此前相关研究有限的历史指纹提取方法。总共180枚指纹被沉积在玻璃表面,进行增强、提取并用于DNA分型处理。对于所有测试的粉末和提取方法,以及修饰过的指纹和自然指纹,均能提取DNA并进行分型,回收的分型百分比无显著差异。从有一个或多个等位基因被检测到的历史指纹提取物中(72次提取物中的79.2%)也能获得DNA分型。这些结果证明了不同粉末/提取组合与DNA分型之间的兼容性,从而提高了法医案件中指纹的证据价值。